Arid
DOI10.3390/fermentation7040280
Selection of Three Indigenous Lebanese Yeast Saccharomyces cerevisiae with Physiological Traits from Grape Varieties in Western Semi-Desert and Pedoclimatic Conditions in the Bekaa Valley
El Dana, Fatima; Hayar, Salem; Colosio, Marie-Charlotte
通讯作者Hayar, S (corresponding author),Lebanese Univ, Doctoral Sch Sci & Technol, Res Platform Environm Sci EDST PRASE, Rafic Hariri Campus, Hadath, Mt Lebanon, Lebanon. ; Hayar, S (corresponding author),Lebanese Univ, Fac Agr Engn & Vet Med, Dept Plant Protect, Beirut, Lebanon.
来源期刊FERMENTATION-BASEL
EISSN2311-5637
出版年2021
卷号7期号:4
英文摘要Wine production depends on the fermentation process performed by yeasts, especially (but not solely) strains of the species Saccharomyces cerevisiae, which is a technique that has been practiced from the Middle Ages till modern days. Selecting indigenous starters offers a beneficial technique to manage alcoholic grape juice fermentation, conserving the particular sensory qualities of wine produced from specific regions. This paper investigated yeast biodiversity of four grape varieties (Carignan, Syrah, Grenache, and Aswad Karesh) grown in the pedoclimatic western semi-desert Bekaa Valley. Further research identified, characterized, and selected strains with the most industrial wine interest and economic value to Lebanon. By using molecular methods and by the ITS PCR analysis, the isolates belonging to the Saccharomyces and non-Saccharomyces genus were identified. These isolates taken from four varieties were further characterized by amplification with Interdelta and delta 12/delta 21 primer pairs, permitting the identification of 96 S. cerevisiae strains. Forty-five genomically homogenous groups were classified through the comparison between their mtDNA RFLP patterns. Based on physiological characterization analysis (H2S and SO2 production, killer phenotype, sugar consumption, malic and acetic acid, etc.), three strains (NL28629, NL28649, and NL28652) showed interesting features, where they were also vigorously fermented in a synthetic medium. These strains can be used as a convenient starter for typical wine production. In particular, Carignan and Syrah had the highest percentage of strains with the most desirable physiological parameters.
英文关键词Saccharomyces cerevisiae molecular identification physiological characterization inoculated fermentation PCR yeast selection Lebanese vineyard
类型Article
语种英语
开放获取类型gold
收录类别SCI-E
WOS记录号WOS:000737120900001
WOS关键词RIBOSOMAL-RNA GENE ; WINE FERMENTATION ; POTENTIAL IMPACT ; CLIMATE-CHANGE ; RFLP ANALYSIS ; STRAINS ; IDENTIFICATION ; QUALITY ; REGION ; PCR
WOS类目Biotechnology & Applied Microbiology ; Food Science & Technology
WOS研究方向Biotechnology & Applied Microbiology ; Food Science & Technology
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/375779
作者单位[El Dana, Fatima] Lebanese Univ, Fac Sci, Sect 1, Hadath, Mt Lebanon, Lebanon; [El Dana, Fatima; Hayar, Salem] Lebanese Univ, Doctoral Sch Sci & Technol, Res Platform Environm Sci EDST PRASE, Rafic Hariri Campus, Hadath, Mt Lebanon, Lebanon; [Hayar, Salem] Lebanese Univ, Fac Agr Engn & Vet Med, Dept Plant Protect, Beirut, Lebanon; [Colosio, Marie-Charlotte] Inst Francais Vigne & Vin IFV, F-44120 Vertou, France
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El Dana, Fatima,Hayar, Salem,Colosio, Marie-Charlotte. Selection of Three Indigenous Lebanese Yeast Saccharomyces cerevisiae with Physiological Traits from Grape Varieties in Western Semi-Desert and Pedoclimatic Conditions in the Bekaa Valley[J],2021,7(4).
APA El Dana, Fatima,Hayar, Salem,&Colosio, Marie-Charlotte.(2021).Selection of Three Indigenous Lebanese Yeast Saccharomyces cerevisiae with Physiological Traits from Grape Varieties in Western Semi-Desert and Pedoclimatic Conditions in the Bekaa Valley.FERMENTATION-BASEL,7(4).
MLA El Dana, Fatima,et al."Selection of Three Indigenous Lebanese Yeast Saccharomyces cerevisiae with Physiological Traits from Grape Varieties in Western Semi-Desert and Pedoclimatic Conditions in the Bekaa Valley".FERMENTATION-BASEL 7.4(2021).
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