干旱区生态环境知识资源中心(Arid): 三文鱼中晚期糖基化终末产物含量测定及产地特征因子分析

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	三文鱼中晚期糖基化终末产物含量测定及产地特征因子分析
其他题名	Determination of Advanced Glycation End Products in Salmon and Exploration of Characteristic Indicators to Identify Its Geographical Origin
	徐正华; 吴家丽; 朱克卫; 梁玉燊; 杨雪娇; 赵勇; 凌菁; 郑思珩; 周衡刚; 张昆; 朱玉珍; 曾茂茂
来源期刊	食品科学
ISSN	1002-6630
出版年	2021
卷号	42 期号:8 页码:1002-6630
中文摘要	建立超高效液相色谱-串联三重四极杆质谱同时检测三文鱼中羧甲基赖氨酸(N-carboxymethyllysine, CML)和羧乙基赖氨酸(N-carboxyethyllysine,CEL)的分析方法。色谱柱为ACQUITY UPLC HSS T3柱(2.1 mm*150 mm,1.8 mum),流动相A为100%乙腈、B为0.1%甲酸,电喷雾离子源正离子模式扫描,多反应监测模式定性分析,同位素内标稀释法定量;样品经脱脂、还原、水解和Oasis MCX固相萃取柱净化等步骤后上机分析;方法平均回收率为90%~95%,精密度相对标准偏差小于5%,CEL检出限为0.032 7 ng/g,定量限为0.142 2 ng/g。采用该法对来自挪威、智利和法罗群岛的21份三文鱼样品进行含量测定,并对所获数据进行主成分分析。结果表明,即使是相同品种的三文鱼在不同国家和不同个体之间CML和CEL含量存在很大的生物差异性,并且发现CEL有望成为鉴别挪威三文鱼和智利、法罗群岛三文鱼的产地特征因子。
英文摘要	A method for simultaneous determination of carboxymethyllysine (N-carboxymethyllysine, CML) and carboxyethyllysine (N-carboxyethyllysine, CEL) in salmon by ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was developed in this paper. The chromatographic separation was performed on an ACQUITY UPLC HSS T3 column using a mobile phase composed of 100% acetonitrile and 0.1% formic acid, and the separated analytes were detected by electrospray ionization in the positive ion (ESI+) mode under multiple reaction monitoring (MRM) and quantitated by the isotope internal standard dilution method. Samples were degreased, reduced, and hydrolyzed and the hydrolysate was purified by solid phase extraction on an Oasis MCX column before being injected into the instrument. The average recoveries of the method were 90%-95%, the precision expressed as relative standard deviation (RSD) was less than 5%, the limit of detection (LOD) was 0.032 7 ng/g, and the limit of quantitation (LOQ) was 0.142 2 ng/g. The method was used to detect 21 salmon samples from Norway, Chile and the Faroe Islands, and the obtained data were analyzed by principal component analysis (PCA). The results showed that there were significant differences in CML and CEL contents between different countries and individuals of the same species. This study revealed CEL to be a potential characteristic indicator to distinguish Norway salmon from those from Chilean and the Faroe Islands.
中文关键词	超高效液相色谱-串联三重四极杆质谱 ; 晚期糖基化终末产物 ; 羧甲基赖氨酸 ; 羧乙基赖氨酸 ; 三文鱼
英文关键词	ultra-high performance liquid chromatography-tandem mass spectrometry advanced glycation end products N-carboxymethyllysine N-carboxyethyllysine salmon
类型	Article
语种	中文
国家	中国
收录类别	CSCD
WOS类目	Food Science & Technology
CSCD记录号	CSCD:6968221
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/365278
作者单位	徐正华, 黄埔海关技术中心, 广州, 广东 510770, 中国.; 朱克卫, 黄埔海关技术中心, 广州, 广东 510770, 中国.; 杨雪娇, 黄埔海关技术中心, 广州, 广东 510770, 中国.; 郑思珩, 黄埔海关技术中心, 广州, 广东 510770, 中国.; 周衡刚, 黄埔海关技术中心, 广州, 广东 510770, 中国.; 朱玉珍, 黄埔海关技术中心, 广州, 广东 510770, 中国.; 吴家丽, 江南大学, 食品科学与技术国家重点实验室, 无锡, 江苏 214122, 中国.; 梁玉燊, 江南大学, 食品科学与技术国家重点实验室, 无锡, 江苏 214122, 中国.; 凌菁, 江南大学, 食品科学与技术国家重点实验室, 无锡, 江苏 214122, 中国.; 张昆, 江南大学, 食品科学与技术国家重点实验室, 无锡, 江苏 214122, 中国.; 曾茂茂, 江南大学, 食品科学与技术国家重点实验室, 无锡, 江苏 214122, 中国.; 赵勇, 广州海关技术中心, 广州, 广东 510000, 中国.
推荐引用方式 GB/T 7714	徐正华,吴家丽,朱克卫,等. 三文鱼中晚期糖基化终末产物含量测定及产地特征因子分析[J],2021,42(8):1002-6630.
APA	徐正华.,吴家丽.,朱克卫.,梁玉燊.,杨雪娇.,...&曾茂茂.(2021).三文鱼中晚期糖基化终末产物含量测定及产地特征因子分析.食品科学,42(8),1002-6630.
MLA	徐正华,et al."三文鱼中晚期糖基化终末产物含量测定及产地特征因子分析".食品科学 42.8(2021):1002-6630.