干旱区生态环境知识资源中心(Arid): Scat DNA as a non-invasive method for estimating the abundance of the vulnerable mala (Lagorchestes hirsutus)

Arid

DOI	10.1071/WR22122
	Scat DNA as a non-invasive method for estimating the abundance of the vulnerable mala (Lagorchestes hirsutus)
	Treloar, Shannon; Lohr, Cheryl; Hopkins, Anna J. M.; Ottewell, Kym; McArthur, Shelley; Davis, Robert A.
通讯作者	Treloar, S
来源期刊	WILDLIFE RESEARCH
ISSN	1035-3712
EISSN	1448-5494
出版年	2024
卷号	51 期号:1
英文摘要	Context. Population-monitoring programs often use direct (e.g. live capture or spotlighting) or indirect (e.g. scats sightings) observations to estimate population abundance. Such methods, however, are often inadequate for rare, elusive, or cryptic species due to the difficulty in achieving sufficient encounters or detection rates. The mala (Lagorchestes hirsutus), a small native Australian macropod, listed as Vulnerable by the IUCN, is difficult to capture, susceptible to capture myopathy, and not easily sighted in their dense habitat; consequently, the population size cannot always be estimated. The use of molecular markers to identify individual genotypes from non-invasively collected samples is increasingly being used in wildlife conservation and may be an alternative approach for mala. Aim. The aim of this study was to evaluate the efficacy of noninvasive scat DNA sampling to estimate the population abundance of mala. Methods. A panel of microsatellite markers was developed for the identification of individual mala via profiling of their scats. Scats were systematically collected from a wild mala population located in an 1100-ha fenced reserve in Western Australia. Individual genotypes were determined using the microsatellite markers, and the abundance of mala was estimated using the genotypes with spatially explicit capture-recapture (SECR) and mark-resight analyses. Key results. The genetic markers proved variable and with sufficient exclusionary power to confidently identify unique individuals (mean locus genotyping error rate: 3.1%). Individual genetic identification from scat sampling, when used with traditional mark-recapture/resight analytical models, provides feasible estimates of population abundance. This is the first reliable abundance estimate of this mala population, suggesting a > 70% increase in population size since the initial reintroduction of 64 individuals in 2011-13. Conclusions. Given the inherent difficulties in surveying mala, this approach would be valuable to ensure effective monitoring of the few remaining fenced and island mala populations to prevent further decline of this vulnerable species. Implications. This is the first study to identify species-specific microsatellite markers for mala and use genetic-capture sampling with scat DNA to estimate the abundance of a mala population. The study provides an evaluation of a valuable species monitoring technique that can be applied to other rare, elusive, or cryptic threatened species.
英文关键词	abundance arid zone cryptic species eDNA environmental DNA fenced reserve mala scat
类型	Article
语种	英语
开放获取类型	hybrid
收录类别	SCI-E
WOS记录号	WOS:000952901800001
WOS关键词	ESTIMATING POPULATION-SIZE ; FECAL-DNA ; GENOTYPING ERRORS ; GENETIC-ANALYSIS ; ELUSIVE ANIMALS ; MARK-RECAPTURE ; VULPES-VULPES ; DENSITY ; FOREST ; MICROSATELLITES
WOS类目	Ecology ; Zoology
WOS研究方向	Environmental Sciences & Ecology ; Zoology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/405942
推荐引用方式 GB/T 7714	Treloar, Shannon,Lohr, Cheryl,Hopkins, Anna J. M.,et al. Scat DNA as a non-invasive method for estimating the abundance of the vulnerable mala (Lagorchestes hirsutus)[J],2024,51(1).
APA	Treloar, Shannon,Lohr, Cheryl,Hopkins, Anna J. M.,Ottewell, Kym,McArthur, Shelley,&Davis, Robert A..(2024).Scat DNA as a non-invasive method for estimating the abundance of the vulnerable mala (Lagorchestes hirsutus).WILDLIFE RESEARCH,51(1).
MLA	Treloar, Shannon,et al."Scat DNA as a non-invasive method for estimating the abundance of the vulnerable mala (Lagorchestes hirsutus)".WILDLIFE RESEARCH 51.1(2024).