干旱区生态环境知识资源中心(Arid): Isolation and propagation of an Egyptian Theileria annulata infected cell line and evaluation of its use as a vaccine to protect cattle against field challenge

Arid

DOI	10.1038/s41598-024-57325-2
	Isolation and propagation of an Egyptian Theileria annulata infected cell line and evaluation of its use as a vaccine to protect cattle against field challenge
	AL-Hosary, Amira; Radwan, Ahmed M.; Ahmed, Laila S.; Abdelghaffar, Sary Kh.; Fischer, Susanne; Nijhof, Ard M.; Clausen, Peter-Henning; Ahmed, Jabbar S.
通讯作者	AL-Hosary, A
来源期刊	SCIENTIFIC REPORTS
ISSN	2045-2322
出版年	2024
卷号	14 期号:1
英文摘要	Tropical theileriosis is an important protozoan tick-borne disease in cattle. Vaccination using attenuated schizont-infected cell lines is one of the methods used for controlling the disease. This study describes the production of attenuated schizont-infected cell lines from Egypt and an evaluation of its use as a vaccine to protect calves against clinical disease upon field challenge. Two groups of exotic and crossbred male calves were divided into vaccinated and control groups. The vaccinated groups were inoculated with 4 ml (1 x 10(6) cells/ml) of the attenuated cell line. Three weeks after vaccination, calves of both groups were transported to the New Valley Governorate (Egyptian oasis) where they were kept under field conditions and exposed to the natural Theileria annulata challenge. All animals in the control group showed severe clinical signs and died despite treatment with buparvaquone, which was administered after two days of persistent fever due to a severe drop in packed cell volume (PCV). Animals in the vaccinated group became seropositive without developing severe clinical signs other than transient fever. Post-mortem examinations revealed enlarged and fragile lymph nodes, spleen, and liver with necrosis and hemorrhages. These findings indicate that the Egyptian attenuated cell line was successful in protecting both exotic and crossbred animals against tropical theileriosis under field conditions.
英文关键词	Theileria annulata Cell line Cattle Vaccine Egypt
类型	Article
语种	英语
开放获取类型	Green Published, Green Submitted, gold
收录类别	SCI-E
WOS记录号	WOS:001201873900050
WOS关键词	TROPICAL THEILERIOSIS ; CULTURE VACCINE ; CALVES ; IMMUNIZATION ; RESISTANCE ; DIAGNOSIS ; IMMUNITY ; TICKS
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/405614
推荐引用方式 GB/T 7714	AL-Hosary, Amira,Radwan, Ahmed M.,Ahmed, Laila S.,et al. Isolation and propagation of an Egyptian Theileria annulata infected cell line and evaluation of its use as a vaccine to protect cattle against field challenge[J],2024,14(1).
APA	AL-Hosary, Amira.,Radwan, Ahmed M..,Ahmed, Laila S..,Abdelghaffar, Sary Kh..,Fischer, Susanne.,...&Ahmed, Jabbar S..(2024).Isolation and propagation of an Egyptian Theileria annulata infected cell line and evaluation of its use as a vaccine to protect cattle against field challenge.SCIENTIFIC REPORTS,14(1).
MLA	AL-Hosary, Amira,et al."Isolation and propagation of an Egyptian Theileria annulata infected cell line and evaluation of its use as a vaccine to protect cattle against field challenge".SCIENTIFIC REPORTS 14.1(2024).