干旱区生态环境知识资源中心(Arid): Exploring the dichotomy: Shotgun metagenomics reveals diversity of beneficial and pathogenic protist community in arid wetlands of northeastern South Africa

Arid

DOI	10.1016/j.scitotenv.2024.174306
	Exploring the dichotomy: Shotgun metagenomics reveals diversity of beneficial and pathogenic protist community in arid wetlands of northeastern South Africa
	Ogola, Henry Joseph Oduor; Ijoma, Grace Nkechinyere; Edokpayi, Joshua Nosa
通讯作者	Ogola, HJO
来源期刊	SCIENCE OF THE TOTAL ENVIRONMENT
ISSN	0048-9697
EISSN	1879-1026
出版年	2024
卷号	946
英文摘要	Arid regions harbor seasonal and permanent wetlands, as biodiversity hotspots crucial for ecosystem services despite harsh conditions. These wetlands, typically dependent on episodic intense rainfall, are understudied compared to their humid counterparts. While the diversity of plants and animals in these wetlands is well-known, the microbial communities remain largely unexplored. To address this knowledge gap, we employed metagenome sequencing technologies to profile protist communities, including pathogenic protozoa, and their associated functional pathways, in sediment of permanent and seasonal arid freshwater wetlands across northern South Africa. Results revealed a core community of protists dominated by phylum Apicomplexa (66.73 %), Euglenazoa (19.03 %), Bacillariophyta (5.44 %), Metamonada (4.65 %), Cryptophyta (1.90 %), and Amoebazoa (1.21 %). Seasonal wetlands showed significantly higher protist diversity compared to permanent wetlands (Shannon index, p = 0.019; Chao1, p = 0.0095). A high abundance and diversity of human and zoonotic pathogenic protists (87.67 %) was observed, with lower levels of photoautotrophs (6.69 %) and limited diversity of phagotrophs (5.64 %). Key photoautotrophs identified included diatoms (Thalassiosiraceae and Phaeodactylaceae) and cryptophytes (genus Hemiselmis and Cryptophyta), with consumers/phagotrophs exhibited a correlation with the bacterial community abundance (r(2) = 0.218, p < 0.001). Pathogenic protozoans identified, include malaria-causing Plasmodium, kinetoplastids (genus Besnoita, Theilleria, Neospora, Toxoplasma, Encephalitozoon, and Babesia) and waterborne protozoans of public health importance (such as Cryptosporidium parvum and Giardia lamblia). Furthermore, the enrichment of pathogenesis-associated pathways (amino acid biosynthesis, peptidoglycan maturation, heme biosynthesis and degradation, and the Calvin-Benson-Bassham cycle), along with virulence gene families identified, highlighted these wetlands as potential reservoirs for infectious diseases. Our results unveil a baseline protist taxonomic and functional composition within arid wetlands, including beneficial and pathogenic protozoa. The close proximity of these wetlands to human activity raises concern for local and transboundary spread of these pathogens. Thus, continued monitoring is vital for disease control and preserving these unique ecosystems.
英文关键词	Pathogenic protozoa Public health Arid wetlands Shotgun metagenomics Disease reservoirs Phagotrophs Diatoms
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:001280875000001
WOS关键词	LIMPOPO PROVINCE ; SCHOOL-CHILDREN ; VHEMBE DISTRICT ; EPIDEMIOLOGY ; PREVALENCE ; INFECTION
WOS类目	Environmental Sciences
WOS研究方向	Environmental Sciences & Ecology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/405520
推荐引用方式 GB/T 7714	Ogola, Henry Joseph Oduor,Ijoma, Grace Nkechinyere,Edokpayi, Joshua Nosa. Exploring the dichotomy: Shotgun metagenomics reveals diversity of beneficial and pathogenic protist community in arid wetlands of northeastern South Africa[J],2024,946.
APA	Ogola, Henry Joseph Oduor,Ijoma, Grace Nkechinyere,&Edokpayi, Joshua Nosa.(2024).Exploring the dichotomy: Shotgun metagenomics reveals diversity of beneficial and pathogenic protist community in arid wetlands of northeastern South Africa.SCIENCE OF THE TOTAL ENVIRONMENT,946.
MLA	Ogola, Henry Joseph Oduor,et al."Exploring the dichotomy: Shotgun metagenomics reveals diversity of beneficial and pathogenic protist community in arid wetlands of northeastern South Africa".SCIENCE OF THE TOTAL ENVIRONMENT 946(2024).