干旱区生态环境知识资源中心(Arid): The effects of climate and soil depth on living and dead bacterial communities along a longitudinal gradient in Chile

Arid

DOI	10.1016/j.scitotenv.2024.173846
	The effects of climate and soil depth on living and dead bacterial communities along a longitudinal gradient in Chile
	Wang, Xiuling; Ganzert, Lars; Bartholomaeus, Alexander; Amen, Rahma; Yang, Sizhong; Guzman, Carolina Merino; Matus, Francisco; Albornoz, Maria Fernanda; Aburto, Felipe; Oses-Pedraza, Romulo; Friedl, Thomas; Wagner, Dirk
通讯作者	Wagner, D
来源期刊	SCIENCE OF THE TOTAL ENVIRONMENT
ISSN	0048-9697
EISSN	1879-1026
出版年	2024
卷号	945
英文摘要	Soil bacterial communities play a critical role in shaping soil stability and formation, exhibiting a dynamic interaction with local climate and soil depth. We employed an innovative DNA separation method to characterize microbial assemblages in low -biomass environments such as deserts and distinguish between intracellular DNA (iDNA) and extracellular DNA (eDNA) in soils. This approach, combined with analyses of physicochemical properties and co -occurrence networks, investigated soil bacterial communities across four sites representing diverse climatic gradients (i.e., arid, semi -arid, Mediterranean, and humid) along the Chilean Coastal Cordillera. The separation method yielded a distinctive unimodal pattern in the iDNA pool alpha diversity, increasing from arid to semi -arid climates and decreasing in humid environments, highlighting the rapid feedback of the iDNA community to increasing soil moisture. In the arid region, harsh surface conditions restrict bacterial growth, leading to peak iDNA abundance and diversity occurring in slightly deeper layers than the other sites. Our findings confirmed the association between specialist bacteria and ecosystem -functional traits. We observed transitions from Halomonas and Delftia , resistant to extreme arid environments, to Class AD3 and the genus Bradyrhizobium , associated with plants and organic matter in humid environments. The distance -based redundancy analysis (dbRDA) analysis revealed that soil pH and moisture were the key parameters that influenced bacterial community variation. The eDNA community correlated slightly better with the environment than the iDNA community. Soil depth was found to influence the iDNA community significantly but not the eDNA community, which might be related to depth -related metabolic activity. Our investigation into iDNA communities uncovered deterministic community assembly and distinct co -occurrence modules correlated with unique bacterial taxa, thereby showing connections with sites and key environmental factors. The study additionally revealed the effects of climatic gradients and soil depth on living and dead bacterial communities, emphasizing the need to distinguish between iDNA and eDNA pools.
英文关键词	Deep soil bacterial community Microbial diversity Climate gradient 16S rRNA sequencing Extracellular DNA (eDNA) Intracellular DNA (iDNA)
类型	Article
语种	英语
开放获取类型	hybrid
收录类别	SCI-E
WOS记录号	WOS:001260609000001
WOS关键词	EXTRACELLULAR DNA ; SEDIMENT ; VEGETATION ; DIVERSITY ; RESPONSES ; SALINITY ; PROFILES ; DYNAMICS ; MOISTURE ; HABITAT
WOS类目	Environmental Sciences
WOS研究方向	Environmental Sciences & Ecology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/405513
推荐引用方式 GB/T 7714	Wang, Xiuling,Ganzert, Lars,Bartholomaeus, Alexander,et al. The effects of climate and soil depth on living and dead bacterial communities along a longitudinal gradient in Chile[J],2024,945.
APA	Wang, Xiuling.,Ganzert, Lars.,Bartholomaeus, Alexander.,Amen, Rahma.,Yang, Sizhong.,...&Wagner, Dirk.(2024).The effects of climate and soil depth on living and dead bacterial communities along a longitudinal gradient in Chile.SCIENCE OF THE TOTAL ENVIRONMENT,945.
MLA	Wang, Xiuling,et al."The effects of climate and soil depth on living and dead bacterial communities along a longitudinal gradient in Chile".SCIENCE OF THE TOTAL ENVIRONMENT 945(2024).