干旱区生态环境知识资源中心(Arid): Regulation of nitrite-dependent anaerobic methane oxidation bacteria by available phosphorus and microbial communities in lake sediments of cold and arid regions

Arid

DOI	10.1016/j.scitotenv.2024.172065
	Regulation of nitrite-dependent anaerobic methane oxidation bacteria by available phosphorus and microbial communities in lake sediments of cold and arid regions
	Ren, Zixuan; Li, Yingnan; Yin, Jiahui; Zhao, Ziwen; Hu, Nan; Zhao, Manping; Wang, Yongman; Wang, Lixin; Wu, Linhui
通讯作者	Wu, LH
来源期刊	SCIENCE OF THE TOTAL ENVIRONMENT
ISSN	0048-9697
EISSN	1879-1026
出版年	2024
卷号	926
英文摘要	As global anthropogenic nitrogen inputs continue to rise, nitrite-dependent anaerobic methane oxidation (NDAMO) plays an increasingly significant role in CH4 consumption in lake sediments. However, there is a dearth of knowledge regarding the effects of anthropogenic activities on N-DAMO bacteria in lakes in the cold and arid regions. Sediment samples were collected from five sampling areas in Lake Ulansuhai at varying depth ranges (0-20, 20-40, and 40-60 cm). The ecological characterization and niche differentiation of N-DAMO bacteria were investigated using bioinformatics and molecular biology techniques. Quantitative PCR confirmed the presence of N-DAMO bacteria in Lake Ulansuhai sediments, with 16S rRNA gene abundances ranging from 1.72 x 10(4) to 5.75 x 10(5) copies.g(-1) dry sediment. The highest abundance was observed at the farmland drainage outlet with high available phosphorus (AP). Anthropogenic disturbances led to a significant increase in the abundance of N-DAMO bacteria, though their diversity remained unaffected. The heterogeneous community of N-DAMO bacteria was affected by interactions among various environmental characteristics, with AP and oxidation-reduction potential identified as the key drivers in this study. The Mantel test indicated that the NDAMO bacterial abundance was more readily influenced by the presence of the denitrification genes (nirS and nirK). Network analysis revealed that the community structure of N-DAMO bacteria generated numerous links (especially positive links) with microbial taxa involved in carbon and nitrogen cycles, such as methanogens and nitrifying bacteria. In summary, N-DAMO bacteria exhibited sensitivity to both environmental and microbial factors under various human disturbances. This study provides valuable insights into the distribution patterns N-DAMO bacteria and their roles in nitrogen and carbon cycling within lake ecosystems.
英文关键词	Anaerobic oxidation of methane Lake in cold-arid region Environmental factors Functional microorganisms Anthropogenic disturbance
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:001223980700001
WOS关键词	OXIDIZING BACTERIA ; DIVERSITY ; QUANTIFICATION ; ENRICHMENT ; NIRK
WOS类目	Environmental Sciences
WOS研究方向	Environmental Sciences & Ecology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/405480
推荐引用方式 GB/T 7714	Ren, Zixuan,Li, Yingnan,Yin, Jiahui,et al. Regulation of nitrite-dependent anaerobic methane oxidation bacteria by available phosphorus and microbial communities in lake sediments of cold and arid regions[J],2024,926.
APA	Ren, Zixuan.,Li, Yingnan.,Yin, Jiahui.,Zhao, Ziwen.,Hu, Nan.,...&Wu, Linhui.(2024).Regulation of nitrite-dependent anaerobic methane oxidation bacteria by available phosphorus and microbial communities in lake sediments of cold and arid regions.SCIENCE OF THE TOTAL ENVIRONMENT,926.
MLA	Ren, Zixuan,et al."Regulation of nitrite-dependent anaerobic methane oxidation bacteria by available phosphorus and microbial communities in lake sediments of cold and arid regions".SCIENCE OF THE TOTAL ENVIRONMENT 926(2024).