干旱区生态环境知识资源中心(Arid): In vitro regeneration of immature zygotic embryos of Melia Volkensii Gürke for accelerated breeding

Arid

DOI	10.1007/s11240-023-02615-2
	In vitro regeneration of immature zygotic embryos of Melia Volkensii Gürke for accelerated breeding
	Kimani, Priscilla N.; Wamalwa, Lydia N.; Ngugi, Kahiu; Omondi, Stephen F.; Machua, Joseph M.; Magomere, Titus; Mulatya, Jackson; Werbrouck, Stefaan P. O.
通讯作者	Werbrouck, SPO
来源期刊	PLANT CELL TISSUE AND ORGAN CULTURE
ISSN	0167-6857
EISSN	1573-5044
出版年	2024
卷号	156 期号:1
英文摘要	Melia volkensii is a dryland mahogany tree species in East Africa that can be harvested for timber in 15 years and has potential for commercial planting in arid and semi-arid areas. The current breeding programme focusing on growth rate, growth habit and shape is delayed by the long juvenile period and slow seed development. This study evaluated early embryo regeneration as a way to circumvent slow seed maturation. Sixty days after pollination (DAP), zygotic embryos were isolated from immature fruits and transferred to Murashige & Skoog (MS) medium without plant growth regulator (PGR) or with different concentrations of 6-benzylaminopurine (BAP), thidiazuron (TDZ), indole-3-acetic acid (IAA) and a combination of TDZ and IAA. After 147 days, survival ranged from 2 to 44%. Embryos on PGR-free media developed thick fleshy shoots. All treatments developed callus to varying degrees and TDZ induced somatic embryos that subsequently developed into shoot clusters. Microshoots also developed from non-embryogenic callus on media containing 1.5 mg/l BAP and directly on media containing IAA. These shoots developed further on hormone-free media and were then rooted on media containing indole-3-butyric acid (IBA). Overall, seedlings were regenerated in 207 days after pollination, compared with the natural seed development period of nearly 390 days, yielding a potential time saving of 47% for progeny generation. This study demonstrates that early embryo regeneration can not only shorten the breeding process, but also rapidly produce many clones so that their value can be tested immediately in different environments.
英文关键词	Melia Volkensii Regeneration Immature zygotic embryos Somatic embryogenesis Breeding
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:001123928600004
WOS关键词	SOMATIC EMBRYOGENESIS ; PLANT-REGENERATION ; CULTURE ; THIDIAZURON ; EFFICIENT ; ROOT
WOS类目	Biotechnology & Applied Microbiology ; Plant Sciences
WOS研究方向	Biotechnology & Applied Microbiology ; Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/405100
推荐引用方式 GB/T 7714	Kimani, Priscilla N.,Wamalwa, Lydia N.,Ngugi, Kahiu,et al. In vitro regeneration of immature zygotic embryos of Melia Volkensii Gürke for accelerated breeding[J],2024,156(1).
APA	Kimani, Priscilla N..,Wamalwa, Lydia N..,Ngugi, Kahiu.,Omondi, Stephen F..,Machua, Joseph M..,...&Werbrouck, Stefaan P. O..(2024).In vitro regeneration of immature zygotic embryos of Melia Volkensii Gürke for accelerated breeding.PLANT CELL TISSUE AND ORGAN CULTURE,156(1).
MLA	Kimani, Priscilla N.,et al."In vitro regeneration of immature zygotic embryos of Melia Volkensii Gürke for accelerated breeding".PLANT CELL TISSUE AND ORGAN CULTURE 156.1(2024).