干旱区生态环境知识资源中心(Arid): Stimulation of organic N mineralization by N-acquiring enzyme activity alleviates soil microbial N limitation following afforestation in subtropical karst areas

Arid

DOI	10.1007/s11104-024-06668-w
	Stimulation of organic N mineralization by N-acquiring enzyme activity alleviates soil microbial N limitation following afforestation in subtropical karst areas
	Liu, Lijun; Zhu, Qilin; Wen, Dongni; Yang, Lin; Ni, Kang; Xu, Xingliang; Cao, Jianhua; Meng, Lei; Yang, Jinling; Zhou, Jinxing; Zhu, Tongbin; Mueller, Christoph
通讯作者	Zhu, TB
来源期刊	PLANT AND SOIL
ISSN	0032-079X
EISSN	1573-5036
出版年	2024
英文摘要	Aims Stoichiometry of soil extracellular enzymes such as the ratios of carbon (C)-, nitrogen (N)-, and phosphorus (P)-acquiring enzymes as well as their vector length and angle are used to reveal the biogeochemical equilibrium between microbial nutrient requirements and soil nutrient availability. However, the dynamics of soil extracellular enzymes activities and microbial N limitation following afforestation remain poorly understood in karst rocky desertification areas. Methods Soil samples were collected from Dodonaea viscosa plantations after 0, 10, 20, and 40 years of afforestation following the abandonment of croplands in a karst rocky desertification area, and a natural restored shrubland soil was served as the control. The activities of C, N, and P extracellular enzymes were measured and the stoichiometric and vector ratios of extracellular enzymes were calculated to quantify microbial nutrient limitation. Results The stoichiometric ratio of soil C: N:P acquisition enzymes was 0.63:1.48:1.0, with a vector angle of 34.7 in croplands, indicating high microbial N limitation. Compared to the croplands, D. viscosa afforestation significantly increased soil C-, N-, and P-acquiring enzyme activities and gradually increased C: N:P stoichiometric ratio and vector angle to 0.75:1.39:1-0.76:1.17:1 and 34.9-38.2, respectively, indicating that afforestation alleviated microbial N limitation. Furthermore, gross N mineralization and gross ammonium immobilization rates increased by 140-278% and 340-801% following afforestation due to the increase in soil organic C and total N contents and the > 2 mm soil aggregates. The vector angle positively correlated with beta-N-acetylglucosaminidase activity, gross N mineralization, and gross ammonium immobilization. Notably, the vector angle of extracellular enzymes following 40-year afforestation was still 6.63% lower than that of the shrubland soil. Conclusion Our results suggested that afforestation could substantially increase gross N mineralization through stimulating beta-N-acetylglucosaminidase activity and gross ammonium immobilization, thereby reducing microbial N limitation. However, this limitation persists even following long-term afforestation in karst rocky desertification areas.
英文关键词	Karst Vegetation restoration Soil extracellular enzyme stoichiometry Microbial N limitation Mineralization
类型	Article ; Early Access
语种	英语
收录类别	SCI-E
WOS记录号	WOS:001201750000002
WOS关键词	ECOENZYMATIC STOICHIOMETRY ; NITROGEN TRANSFORMATIONS ; NUTRIENT ACQUISITION ; RETENTION CAPACITY ; MICROORGANISMS ; CARBON ; ACCUMULATION ; RECOVERY ; NITRATE ; MATTER
WOS类目	Agronomy ; Plant Sciences ; Soil Science
WOS研究方向	Agriculture ; Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/405094
推荐引用方式 GB/T 7714	Liu, Lijun,Zhu, Qilin,Wen, Dongni,et al. Stimulation of organic N mineralization by N-acquiring enzyme activity alleviates soil microbial N limitation following afforestation in subtropical karst areas[J],2024.
APA	Liu, Lijun.,Zhu, Qilin.,Wen, Dongni.,Yang, Lin.,Ni, Kang.,...&Mueller, Christoph.(2024).Stimulation of organic N mineralization by N-acquiring enzyme activity alleviates soil microbial N limitation following afforestation in subtropical karst areas.PLANT AND SOIL.
MLA	Liu, Lijun,et al."Stimulation of organic N mineralization by N-acquiring enzyme activity alleviates soil microbial N limitation following afforestation in subtropical karst areas".PLANT AND SOIL (2024).