干旱区生态环境知识资源中心(Arid): Detection and molecular characterization of soft scale insect, Megapulvinaria maxima (Green) (Hemiptera: Coccidae) infesting Gymnema sylvestre (Retz.) R.Br. ex Sm. from India

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DOI	10.1007/s12600-024-01175-1
	Detection and molecular characterization of soft scale insect, Megapulvinaria maxima (Green) (Hemiptera: Coccidae) infesting Gymnema sylvestre (Retz.) R.Br. ex Sm. from India
	Shivakumara, K. T.; Polaiah, A. C.; Joshi, Sunil; Keerthi, M. C.; Manjunatha, C.; Ramya, R. S.; Gotyal, B. S.; Madhu, T. N.
通讯作者	Shivakumara, KT
来源期刊	PHYTOPARASITICA
ISSN	0334-2123
EISSN	1876-7184
出版年	2024
卷号	52 期号:4
英文摘要	Gymnema sylvestre (Retz.) R. Br. ex Sm is a vital medicinal plant effective in treatment of Diabetes mellitus. The soft scale, Megapulvinaria maxima (Green) (Hemiptera: Coccidae), has emerged as a significant pest infesting Gymnema sylvestre in the semi-arid region of India. A random survey revealed infestation on leaves, stems, and fruits, by both nymphs and adults extracting plant sap and secreting excess honeydew. This honeydew adversely impacts leaf quality which is essential for bio-molecule extraction. Early pest detection is crucial for effective management, but the precise morphological identification of scale insects poses challenges. Utilizing morphological keys and sequencing the Cytochrome c oxidase subunit I (COI) gene (GenBank No. OR602704), we identified the scale insects as M. maxima. Evolutionary divergence studies and phylogenetic analysis indicated no divergence with the Republic of Korea isolate (MK543913), while with the isolate of the accession number, MW450949 highest evolutionary divergence was exhibited (0.72%). The isolates (KP189890) and (KP189893) from China showed less association with the study isolate compared to other populations. The severity of incidence peaked during the 48th and 45th standard meteorological weeks (SMW) in 2019-20 and 2020-21, reaching severity levels of 3.67 and 3.56, respectively. In both years, morning relative humidity showed a significantly positive correlation (r = 0.152 and 0.364*). These findings offer valuable insights for early pest detection and formulating effective management strategies. To the best of our knowledge, this is the first comprehensive study of M. maxima affecting the medicinal herb G. sylvestre in India and globally.
英文关键词	Anti-diabetic Gymnema sylvestre Megapulvinaria maxima Polymerase chain reaction Phylogenetic analysis Severity index
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:001268398500001
WOS类目	Agronomy ; Plant Sciences ; Entomology
WOS研究方向	Agriculture ; Plant Sciences ; Entomology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/405074
推荐引用方式 GB/T 7714	Shivakumara, K. T.,Polaiah, A. C.,Joshi, Sunil,et al. Detection and molecular characterization of soft scale insect, Megapulvinaria maxima (Green) (Hemiptera: Coccidae) infesting Gymnema sylvestre (Retz.) R.Br. ex Sm. from India[J],2024,52(4).
APA	Shivakumara, K. T..,Polaiah, A. C..,Joshi, Sunil.,Keerthi, M. C..,Manjunatha, C..,...&Madhu, T. N..(2024).Detection and molecular characterization of soft scale insect, Megapulvinaria maxima (Green) (Hemiptera: Coccidae) infesting Gymnema sylvestre (Retz.) R.Br. ex Sm. from India.PHYTOPARASITICA,52(4).
MLA	Shivakumara, K. T.,et al."Detection and molecular characterization of soft scale insect, Megapulvinaria maxima (Green) (Hemiptera: Coccidae) infesting Gymnema sylvestre (Retz.) R.Br. ex Sm. from India".PHYTOPARASITICA 52.4(2024).