干旱区生态环境知识资源中心(Arid): Discovering the regulators of heat stress tolerance in Ziziphus nummularia (Burm.f) wight and walk.-arn

Arid

DOI	10.1007/s12298-024-01431-y
	Discovering the regulators of heat stress tolerance in Ziziphus nummularia (Burm.f) wight and walk.-arn
	Panzade, Kishor Prabhakar; Tribhuvan, Kishor U.; Pawar, Deepak V.; Jasrotia, Rahul Singh; Gaikwad, Kishor; Dalal, Monika; Kumar, Ranjeet Ranjan; Singh, Madan Pal; Awasthi, Om Prakash; Padaria, Jasdeep Chatrath
通讯作者	Padaria, JC
来源期刊	PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS
ISSN	0971-5894
EISSN	0974-0430
出版年	2024
卷号	30 期号:3 页码:497-511
英文摘要	Ziziphus nummularia an elite heat-stress tolerant shrub, grows in arid regions of desert. However, its molecular mechanism responsible for heat stress tolerance is unexplored. Therefore, we analysed whole transcriptome of Jaisalmer (heat tolerant) and Godhra (heat sensitive) genotypes of Z. nummularia to understand its molecular mechanism responsible for heat stress tolerance. De novo assembly of 16,22,25,052 clean reads yielded 276,029 transcripts. A total of 208,506 unigenes were identified which contains 4290 and 1043 differentially expressed genes (DEG) in TGO (treated Godhra at 42 degrees C) vs. CGO (control Godhra) and TJR (treated Jaisalmer at 42 degrees C) vs. CJR (control Jaisalmer), respectively. A total of 987 (67 highly enriched) and 754 (34 highly enriched) pathways were obsorved in CGO vs. TGO and CJR vs. TJR, respectively. Antioxidant pathways and TFs like Homeobox, HBP, ARR, PHD, GRAS, CPP, and E2FA were uniquely observed in Godhra genotype and SET domains were uniquely observed in Jaisalmer genotype. Further transposable elements were highly up-regulated in Godhra genotype but no activation in Jaisalmer genotype. A total of 43,093 and 39,278 simple sequence repeats were identified in the Godhra and Jaisalmer genotypes, respectively. A total of 10 DEGs linked to heat stress were validated in both genotypes for their expression under different heat stresses using quantitative real-time PCR. Comparing expression patterns of the selected DEGs identified ClpB1 as a potential candidate gene for heat tolerance in Z. nummularia. Here we present first characterized transcriptome of Z. nummularia in response to heat stress for the identification and characterization of heat stress-responsive genes.
英文关键词	Ziziphus nummularia Heat stress Transcriptome assembly Differential gene expression Transcription factors
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:001190302700001
WOS关键词	RNA-SEQ ANALYSIS ; DIFFERENTIAL EXPRESSION ; GENES ; TRANSCRIPTOME ; PROTEINS ; ARABIDOPSIS ; IMPROVEMENT ; RESPONSES ; DROUGHT ; MARKERS
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/405071
推荐引用方式 GB/T 7714	Panzade, Kishor Prabhakar,Tribhuvan, Kishor U.,Pawar, Deepak V.,et al. Discovering the regulators of heat stress tolerance in Ziziphus nummularia (Burm.f) wight and walk.-arn[J],2024,30(3):497-511.
APA	Panzade, Kishor Prabhakar.,Tribhuvan, Kishor U..,Pawar, Deepak V..,Jasrotia, Rahul Singh.,Gaikwad, Kishor.,...&Padaria, Jasdeep Chatrath.(2024).Discovering the regulators of heat stress tolerance in Ziziphus nummularia (Burm.f) wight and walk.-arn.PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS,30(3),497-511.
MLA	Panzade, Kishor Prabhakar,et al."Discovering the regulators of heat stress tolerance in Ziziphus nummularia (Burm.f) wight and walk.-arn".PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS 30.3(2024):497-511.