干旱区生态环境知识资源中心(Arid): Development of a Quantitative Determination Method for Polysorbate 80 in Biologicals by Size-Exclusion High-Performance Liquid Chromatography

Arid

DOI	10.1007/s11094-024-03110-4
	Development of a Quantitative Determination Method for Polysorbate 80 in Biologicals by Size-Exclusion High-Performance Liquid Chromatography
	Rounova, O. B.; Korotkov, M. G.; Ustinnikova, O. B.
通讯作者	Rounova, OB
来源期刊	PHARMACEUTICAL CHEMISTRY JOURNAL
ISSN	0091-150X
EISSN	1573-9031
出版年	2024
卷号	57 期号:12 页码:2013-2018
英文摘要	Aquantitative determination method for polysorbate 80 in biological medicinal preparations using size-exclusion chromatography with UV detection was developed. OASIS HLB solid-phase extraction cartridges were used to extract polysorbate 80 from formulations with high protein concentrations. The HPLC separation was performed using a TSKgel G2000SWX column in isocratic mode. The mobile phase was phosphate buffer (0.1 M) containing sodium sulfate (0.1 M) and sodium dodecyl sulfate (0.1%). The developed method could be used for quantitative determination of polysorbate 80 in biological medicinal products.
英文关键词	polysorbate 80 size-exclusion high-performance liquid chromatography quantitative determination biological medicinal products
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:001204368800007
WOS关键词	FORMULATIONS ; HPLC ; MS
WOS类目	Chemistry, Medicinal ; Pharmacology & Pharmacy
WOS研究方向	Pharmacology & Pharmacy
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/405051
推荐引用方式 GB/T 7714	Rounova, O. B.,Korotkov, M. G.,Ustinnikova, O. B.. Development of a Quantitative Determination Method for Polysorbate 80 in Biologicals by Size-Exclusion High-Performance Liquid Chromatography[J],2024,57(12):2013-2018.
APA	Rounova, O. B.,Korotkov, M. G.,&Ustinnikova, O. B..(2024).Development of a Quantitative Determination Method for Polysorbate 80 in Biologicals by Size-Exclusion High-Performance Liquid Chromatography.PHARMACEUTICAL CHEMISTRY JOURNAL,57(12),2013-2018.
MLA	Rounova, O. B.,et al."Development of a Quantitative Determination Method for Polysorbate 80 in Biologicals by Size-Exclusion High-Performance Liquid Chromatography".PHARMACEUTICAL CHEMISTRY JOURNAL 57.12(2024):2013-2018.