干旱区生态环境知识资源中心(Arid): Dimerization of hub protein DYNLL1 and bZIP transcription factor CREB3L1 enhances transcriptional activation of CREB3L1 target genes like arginine vasopressin

Arid

DOI	10.1016/j.peptides.2024.171269
	Dimerization of hub protein DYNLL1 and bZIP transcription factor CREB3L1 enhances transcriptional activation of CREB3L1 target genes like arginine vasopressin
	Greenwood, Mingkwan; Gillard, Benjamin T.; Murphy, David; Greenwood, Michael P.
通讯作者	Greenwood, MP
来源期刊	PEPTIDES
ISSN	0196-9781
EISSN	1873-5169
出版年	2024
卷号	179
英文摘要	bZIP transcription factors can function as homodimers or heterodimers through interactions with their disordered coiled-coil domain. Such dimer assemblies are known to influence DNA-binding specificity and/or the recruitment of binding partners, which can cause a functional switch of a transcription factor from being an activator to a repressor. We recently identified the genomic targets of a bZIP transcription factor called CREB3L1 in rat hypothalamic supraoptic nucleus by ChIP-seq. The objective of this study was to investigate the CREB3L1 protein-to-protein interactome of which little is known. For this approach, we created and screened a rat supraoptic nucleus yeast two-hybrid prey library with the bZIP region of rat CREB3L1 as the bait. Our yeast twohybrid approach captured five putative CREB3L1 interacting prey proteins in the supraoptic nucleus. One interactor was selected by bioinformatic analyses for more detailed investigation by co-immunoprecipitation, immunofluorescent cellular localisation, and reporter assays in vitro. Here we identify dimerisation hub protein Dynein Light Chain LC8-Type 1 as a CREB3L1 interacting protein that in vitro enhances CREB3L1 activation of target genes.
英文关键词	Transcription Transcription factor Basic leucine zipper Yeast two -hybrid Supraoptic nucleus Vasopressin
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:001270711100001
WOS关键词	ENDOPLASMIC-RETICULUM STRESS ; NITRIC-OXIDE SYNTHASE ; LIGHT-CHAIN LC8 ; CREB/ATF-FAMILY ; OASIS ; EXPRESSION ; BINDS ; PIN ; INHIBITOR ; INTERACTS
WOS类目	Biochemistry & Molecular Biology ; Endocrinology & Metabolism ; Pharmacology & Pharmacy
WOS研究方向	Biochemistry & Molecular Biology ; Endocrinology & Metabolism ; Pharmacology & Pharmacy
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/405048
推荐引用方式 GB/T 7714	Greenwood, Mingkwan,Gillard, Benjamin T.,Murphy, David,et al. Dimerization of hub protein DYNLL1 and bZIP transcription factor CREB3L1 enhances transcriptional activation of CREB3L1 target genes like arginine vasopressin[J],2024,179.
APA	Greenwood, Mingkwan,Gillard, Benjamin T.,Murphy, David,&Greenwood, Michael P..(2024).Dimerization of hub protein DYNLL1 and bZIP transcription factor CREB3L1 enhances transcriptional activation of CREB3L1 target genes like arginine vasopressin.PEPTIDES,179.
MLA	Greenwood, Mingkwan,et al."Dimerization of hub protein DYNLL1 and bZIP transcription factor CREB3L1 enhances transcriptional activation of CREB3L1 target genes like arginine vasopressin".PEPTIDES 179(2024).