干旱区生态环境知识资源中心(Arid): Enhancing Chickpea Growth through Drought-Tolerant Bacterial Strains Isolated from the Rhizosphere of Aerva javanica

Arid

DOI	10.21162/PAKJAS/24.264
	Enhancing Chickpea Growth through Drought-Tolerant Bacterial Strains Isolated from the Rhizosphere of Aerva javanica
	Fatima, Ishrat; Ahmad, Maqshoof; Hussain, Azhar; Alataway, Abed; Dewidar, Ahmed Z.; Mattar, Mohamed A.
通讯作者	Ahmad, M
来源期刊	PAKISTAN JOURNAL OF AGRICULTURAL SCIENCES
ISSN	0552-9034
EISSN	2076-0906
出版年	2024
卷号	61 期号:1 页码:163-178
英文摘要	Drought stress has a significant challenge to crop growth, reproduction, and yield in arid regions. Microbes associated with desert plants offer a promising solution by enhancing moisture and nutrient uptake, mitigating water stress, and ultimately boosting crop growth and yield. In this study, the bacteria isolated from the rhizosphere of native plant Aerva javanica, found in the Cholistan desert, were assessed for their effectiveness to promote chickpea seedlings growth. A total of 21 droughttolerant rhizobacterial strains were isolated and characterized, with a focus on their ability to produce exopolysaccharide (EPS) and 1-aminocyclopropane-1-carboxylic acid-deaminase (ACC-deaminase) under water -deficit conditions. These conditions were simulated using polyethylene glycol (PEG) 6000 in the growth medium. Notably, strains CD23 and CD21 exhibited the highest EPS production ability, yielding 10.9 and 10.4 mg L-1 of EPS, respectively, under PEG -induced drought stress however, a minute reduction in EPS production was observed under normal conditions. Similarly, ACC-deaminase activity was significant, measuring 3.2 and 3.2 mu M mg( -1) protein for strains CD23 and CD21, respectively. Further assessments were conducted to evaluate the drought tolerance of these strains at various PEG -6000 levels in jar trials. The strains CD23 and CD21 demonstrated promising results in enhancing chickpea growth. Subsequently, through partial gene sequencing of the 16S rRNA, these strains were identified as Acinetobacter sp. strain CD21 (accession number: MW829536) and Klebsiella sp. strain CD23 (accession number MW829537). The production of ACC-deaminase and EPS by these desert rhizobacteria may serve as crucial indicator for the development of inoculants that can promote chickpea growth under water -deficit conditions.
英文关键词	Rhizobacteria arid climate drought tolerance chickpea
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:001189249700015
WOS关键词	PLANT-GROWTH ; DESICCATION ; QUALITY ; SUGARS ; YIELD
WOS类目	Agriculture, Multidisciplinary
WOS研究方向	Agriculture
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/405013
推荐引用方式 GB/T 7714	Fatima, Ishrat,Ahmad, Maqshoof,Hussain, Azhar,et al. Enhancing Chickpea Growth through Drought-Tolerant Bacterial Strains Isolated from the Rhizosphere of Aerva javanica[J],2024,61(1):163-178.
APA	Fatima, Ishrat,Ahmad, Maqshoof,Hussain, Azhar,Alataway, Abed,Dewidar, Ahmed Z.,&Mattar, Mohamed A..(2024).Enhancing Chickpea Growth through Drought-Tolerant Bacterial Strains Isolated from the Rhizosphere of Aerva javanica.PAKISTAN JOURNAL OF AGRICULTURAL SCIENCES,61(1),163-178.
MLA	Fatima, Ishrat,et al."Enhancing Chickpea Growth through Drought-Tolerant Bacterial Strains Isolated from the Rhizosphere of Aerva javanica".PAKISTAN JOURNAL OF AGRICULTURAL SCIENCES 61.1(2024):163-178.