干旱区生态环境知识资源中心(Arid): Insight into possible therapeutic applications of Ephedra transitoria: in-vitro biological, toxicological activities and GC-MS analysis of aerial arpts' extract

Arid

DOI	10.15835/nbha52113442
	Insight into possible therapeutic applications of Ephedra transitoria: in-vitro biological, toxicological activities and GC-MS analysis of aerial arpts' extract
	Younes, Kareem M.; Alheibshy, Fawaz; Alrashiedi, Amal M.; Alwhadani, Lamya A.; Alshammari, Shomokh M.; Alshammari, Asmaa S.; Albalawi, Manawy K.; Almutiri, Turki A.; Aljohani, Ghazi
通讯作者	Younes, KM
来源期刊	NOTULAE BOTANICAE HORTI AGROBOTANICI CLUJ-NAPOCA
ISSN	0255-965X
EISSN	1842-4309
出版年	2024
卷号	52 期号:1
英文摘要	Most Arab nations have arid regions where Ephedra transitoria, a plant, flourishes. It has a variety of medical benefits. It belongs to the Ephedraceae family. The plant's aerial portions were procured from the Hail region of Saudi Arabia, methanol was used to extract bioactive phytochemicals with variable solubility in an efficient manner, and the resulting extract was tested on several cancer cell lines. The extract's cytotoxic potential was measured by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide assay method against standard vinblastine sulfate and for each cell line, the pertinent half maximal inhibitory concentration values were calculated. Additional apoptosis assays were conducted. Also, the antioxidant activity of plant extract was examined using 2, 2-Diphenyl-1-picrylhydrazyl and [2, 2'-azinobis-(3ethylbenzothiazoline-6-sulfonate)] methods compared to ascorbic acid standard. Also, antimicrobial effect of extract was evaluated on different gram positive and gram -negative bacteria by zone inhibition and minimum inhibitory concentration assay methods. GC -MS analysis was further applied to identify major bioactive substances. Extract showed good cytotoxic activity against both lung and liver cancer cells with relevant half maximal inhibitory concentration values comparable to those of standard. Extract resulted in S -phase arrest, primarily via inducing apoptosis mediated through activating caspase-3, Bax, and p53 proteins. Plant extract showed good antibacterial activities against S. aureus, E. coli and B. subtilis. Also, it showed high antioxidant activity with relevant half maximal inhibitory concentration values comparable to those of standard. Some bioactive compounds were separated and identified by GC -MS analysis such as; ephedrine and quercetin. ephedra transitoria extract possess high cytotoxic activity against lung and liver cancer cells via S -phase arrest mediated by activation of apoptotic proteins. It also possesses high antioxidant and antibacterial activities.
英文关键词	antibacterial antioxidant apoptosis cytotoxic Ephedra
类型	Article
语种	英语
开放获取类型	gold
收录类别	SCI-E
WOS记录号	WOS:001196573600047
WOS关键词	ANTIMICROBIAL ACTIVITY ; CHEMICAL-COMPOSITION ; ANTIOXIDANT ACTIVITY ; MEDICINAL-PLANTS ; DESIGN ; SINICA ; CELL
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/404988
推荐引用方式 GB/T 7714	Younes, Kareem M.,Alheibshy, Fawaz,Alrashiedi, Amal M.,et al. Insight into possible therapeutic applications of Ephedra transitoria: in-vitro biological, toxicological activities and GC-MS analysis of aerial arpts' extract[J],2024,52(1).
APA	Younes, Kareem M..,Alheibshy, Fawaz.,Alrashiedi, Amal M..,Alwhadani, Lamya A..,Alshammari, Shomokh M..,...&Aljohani, Ghazi.(2024).Insight into possible therapeutic applications of Ephedra transitoria: in-vitro biological, toxicological activities and GC-MS analysis of aerial arpts' extract.NOTULAE BOTANICAE HORTI AGROBOTANICI CLUJ-NAPOCA,52(1).
MLA	Younes, Kareem M.,et al."Insight into possible therapeutic applications of Ephedra transitoria: in-vitro biological, toxicological activities and GC-MS analysis of aerial arpts' extract".NOTULAE BOTANICAE HORTI AGROBOTANICI CLUJ-NAPOCA 52.1(2024).