干旱区生态环境知识资源中心(Arid): Assessment of physio-biochemical assessment and gene expression analysis of sugarcane genotypes under water stress

Arid

DOI	10.1007/s11033-024-09251-9
	Assessment of physio-biochemical assessment and gene expression analysis of sugarcane genotypes under water stress
	Rani, Varsha; Sengar, R. S.; Chauhan, Chetan
通讯作者	Sengar, RS
来源期刊	MOLECULAR BIOLOGY REPORTS
ISSN	0301-4851
EISSN	1573-4978
出版年	2024
卷号	51 期号:1
英文摘要	Background Sugarcane, an economically important crop cultivated for its unique character of accumulating sucrose into its stalk and the world's major crop according to production quantity. Sugarcane production is negatively influenced by abiotic stresses because it faces all types of environments due to its long-life cycle period. Among the various abiotic stresses, drought is one of the major limiting factors creates obstacle in sugarcane production. Thus, an attempt was made to assess the molecular insights into sugarcane genotypes under water stress. A preliminary screening was done in ten sugarcane genotypes grown under semi-arid region of India through physiological, biochemical and antioxidant responses of these genotypes under two water deficit levels. Methods In the current study, drought was imposed on ten sugarcane genotypes during their formative stage (110 DAP) by depriving them of irrigation. A pot experiment was carried out to see how several commercial sugarcane genotypes responded to water scarcity. Sugarcane received two treatments, the first after 125 days and the second after 140 days. The physio-biochemical and antioxidant responses recorded were RWC, MSI, SCMR, Proline accumulation, SOD, Catalase, Peroxidase and Lipid peroxidation. The significant variations were recorded in responses of all genotypes. On the basis of physio-biochemical, three genotypes Cos 98,014, Cos 13,235 and Colk 14,201 were selected for differential gene expression pattern analysis. The total RNA was isolated and reverse transcribe to cDNA and real time PCR was performed for expression analysis under 10 genes. Results Under drought conditions, all sugarcane genotypes showed significantly decreased RWC, chlorophyll content, and MSI. However, when water was scarce, proline buildup, malondialdehyde (MDA) contents, enzymatic antioxidant activity (CAT, POD, and SOD), and contents all increased dramatically. Finally, in all physiological and biochemical parameters, Co 98,014 genotype displayed superior adaptation responses to drought stress, followed by Co 018, Cos 13,235, and Colk 14,201. For gene expression analysis out of 21 genes, 10 genes were expressed in sugarcane genotypes, in which 7 genes (Shbbx2, Shbbx3, Shbbx4, Shbbx5, Shbbx8, Shbbx15 and Shbbx20) were upregulated and 3 genes (Shbbx1, Shbbx16 and Shbbx17) were downregulated. Conclusion The statistical analysis conducted in this study demonstrated that drought stress had a negative impact on physiological responses, including RWC, SPAD, and MSI, in sugarcane crops. However, it was found that the crops were able to survive in these stress conditions by increasing their biochemical parameters, all while maintaining their growth and function.
英文关键词	Drought Tolerance Abiotic Stress Antioxidant Defense Oxidative Stress Sugarcane Gene Expression
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:001165228800001
WOS关键词	DROUGHT-STRESS ; ANTIOXIDANT RESPONSES ; PHYSIOLOGICAL TRAITS ; SUPEROXIDE-DISMUTASE ; OXIDATIVE STRESS ; WHEAT GENOTYPES ; USE EFFICIENCY ; GENOME-WIDE ; TOLERANCE ; SALINITY
WOS类目	Biochemistry & Molecular Biology
WOS研究方向	Biochemistry & Molecular Biology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/404928
推荐引用方式 GB/T 7714	Rani, Varsha,Sengar, R. S.,Chauhan, Chetan. Assessment of physio-biochemical assessment and gene expression analysis of sugarcane genotypes under water stress[J],2024,51(1).
APA	Rani, Varsha,Sengar, R. S.,&Chauhan, Chetan.(2024).Assessment of physio-biochemical assessment and gene expression analysis of sugarcane genotypes under water stress.MOLECULAR BIOLOGY REPORTS,51(1).
MLA	Rani, Varsha,et al."Assessment of physio-biochemical assessment and gene expression analysis of sugarcane genotypes under water stress".MOLECULAR BIOLOGY REPORTS 51.1(2024).