Knowledge Resource Center for Ecological Environment in Arid Area
| DOI | 10.1093/mmy/myae034 |
| Aflatoxin profiles of Aspergillus flavus isolates in Sudanese fungal rhinosinusitis | |
| Zhou, Shaoqin; Ismail, Mawahib A. I.; Aimanianda, Vishukumar; de Hoog, G. Sybren; Kang, Yingqian; Ahmed, Sarah A. | |
| 通讯作者 | Kang, YQ |
| 来源期刊 | MEDICAL MYCOLOGY
 |
| ISSN | 1369-3786 |
| EISSN | 1460-2709 |
| 出版年 | 2024 |
| 卷号 | 62期号:4 |
| 英文摘要 | Aspergillus flavus is a commonly encountered pathogen responsible for fungal rhinosinusitis (FRS) in arid regions. The species is known to produce aflatoxins, posing a significant risk to human health. This study aimed to investigate the aflatoxin profiles of A. flavus isolates causing FRS in Sudan. A total of 93 clinical and 34 environmental A. flavus isolates were studied. Aflatoxin profiles were evaluated by phenotypic (thin-layer and high-performance chromatography) and genotypic methods at various temperatures and substrates. Gene expression of aflD and aflR was also analyzed. A total of 42/93 (45%) isolates were positive for aflatoxin B1 and AFB2 by HPLC. When the incubation temperature changed from 28 degrees C to 36 degrees C, the number of positive isolates decreased to 41% (38/93). Genetic analysis revealed that 85% (79/93) of clinical isolates possessed all seven aflatoxin biosynthesis-associated genes, while 27% (14/51) of non-producing isolates lacked specific genes (aflD/aflR/aflS). Mutations were observed in aflS and aflR genes across both aflatoxin-producers and non-producers. Gene expression of aflD and aflR showed the highest expression between the 4th and 6th days of incubation on the Sabouraud medium and on the 9th day of incubation on the RPMI (Roswell Park Memorial Institute) medium. Aspergillus flavus clinical isolates demonstrated aflatoxigenic capabilities, influenced by incubation temperature and substrate. Dynamic aflD and aflR gene expression patterns over time enriched our understanding of aflatoxin production regulation. The overall findings underscored the health risks of Sudanese patients infected by this species, emphasizing the importance of monitoring aflatoxin exposure. Aspergillus flavus, mainly causing fungal rhinosinusitis in Sudan, poses health risks due to aflatoxin production. This study revealed diverse levels of aflatoxin and gene expression of clinical isolates by pheno- and genotypic methods, emphasizing the need for vigilant monitoring in the region. |
| 英文关键词 | Aspergillus flavus fungal rhinosinusitis aflatoxin profiles thin-layer and high-performance chromatography gene expression |
| 类型 | Article |
| 语种 | 英语 |
| 开放获取类型 | hybrid, Green Published |
| 收录类别 | SCI-E |
| WOS记录号 | WOS:001207209200001 |
| WOS关键词 | GENE-EXPRESSION ; MOLECULAR CHARACTERIZATION ; STRAINS ; PCR ; CHROMATOGRAPHY ; CONTAMINATION ; TRANSCRIPTION ; FUMIGATUS ; GROWTH |
| WOS类目 | Infectious Diseases ; Mycology ; Veterinary Sciences |
| WOS研究方向 | Infectious Diseases ; Mycology ; Veterinary Sciences |
| 资源类型 | 期刊论文 |
| 条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/404900 |
| 推荐引用方式 GB/T 7714 | Zhou, Shaoqin,Ismail, Mawahib A. I.,Aimanianda, Vishukumar,et al. Aflatoxin profiles of Aspergillus flavus isolates in Sudanese fungal rhinosinusitis[J],2024,62(4). |
| APA | Zhou, Shaoqin,Ismail, Mawahib A. I.,Aimanianda, Vishukumar,de Hoog, G. Sybren,Kang, Yingqian,&Ahmed, Sarah A..(2024).Aflatoxin profiles of Aspergillus flavus isolates in Sudanese fungal rhinosinusitis.MEDICAL MYCOLOGY,62(4). |
| MLA | Zhou, Shaoqin,et al."Aflatoxin profiles of Aspergillus flavus isolates in Sudanese fungal rhinosinusitis".MEDICAL MYCOLOGY 62.4(2024). |
| 条目包含的文件 | 条目无相关文件。 | |||||
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