干旱区生态环境知识资源中心(Arid): A New Abscisic Acid Conjugate, ABA-L-Glutamate, Determined in Different Plant Species by Combined Immunoaffinity Chromatography-Tandem Mass Spectrometry

Arid

DOI	10.1007/s00344-024-11436-2
	A New Abscisic Acid Conjugate, ABA-L-Glutamate, Determined in Different Plant Species by Combined Immunoaffinity Chromatography-Tandem Mass Spectrometry
	Tureckova, Veronika; Oklestkova, Jana; Zukauskaite, Asta; Eyer, Ludek; Novak, Ondrej; Strnad, Miroslav
通讯作者	Turecková, V
来源期刊	JOURNAL OF PLANT GROWTH REGULATION
ISSN	0721-7595
EISSN	1435-8107
出版年	2024
英文摘要	Abscisic acid (ABA) is a phytohormone that occurs in plants at very low concentration (pmol/g fresh weight) and regulates multiple biological processes, including stomatal closure, seed germination, and responses to environmental stresses. In the present study, isolation of ABA, ABA glucosyl ester, and 11 ABA amino acid conjugates from minute quantities of plant tissue (less than 20 mg fresh weight) was achieved using a purification method based on the combination of an Oasis HLB column and an immunoaffinity sorbent. New monoclonal antibodies raised against (+)-cis,trans-ABA conjugated to BSA through its carboxyl group (C1) were characterised by enzyme-linked immunosorbent assay (ELISA) and used for immunoaffinity chromatography (IAC) gel preparation. The use of immunoaffinity purification significantly reduced matrix effects and increased the selectivity and sensitivity of subsequent UHPLC-MS/MS analysis. In addition to (+)-cis,trans-ABA and its glucosyl ester, a new abscisic acid conjugate, ABA-L-glutamate, was isolated by IAC and identified by tandem mass spectrometry in pea (Pisum sativum L.), Lepidium sativum L. and wheat (Triticum aestivum L.) seedlings. However, it was not found in 10-day-old seedlings of Arabidopsis thaliana or water-stressed tobacco (Nicotiana tabacum L.) leaves. Here, the identification of an ABA conjugate with glutamic acid in plants is described for the first time.
英文关键词	Abscisic acid amino acid conjugate (ABA-AA) Enzyme-linked immunosorbent assay (ELISA) Monoclonal antibody (mAb) Immunoaffinity chromatography (IAC) Ultra-high-performance liquid chromatography (UHPLC) Tandem mass spectrometry (MS/MS)
类型	Article ; Early Access
语种	英语
开放获取类型	hybrid
收录类别	SCI-E
WOS记录号	WOS:001295692600002
WOS关键词	PERFORMANCE LIQUID-CHROMATOGRAPHY ; SOLID-PHASE EXTRACTION ; SIGNAL-TRANSDUCTION ; METABOLITES ; ENZYME ; AUXIN ; IMMUNOASSAY ; RADIOIMMUNOASSAY ; BIOSYNTHESIS ; PURIFICATION
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/404658
推荐引用方式 GB/T 7714	Tureckova, Veronika,Oklestkova, Jana,Zukauskaite, Asta,et al. A New Abscisic Acid Conjugate, ABA-L-Glutamate, Determined in Different Plant Species by Combined Immunoaffinity Chromatography-Tandem Mass Spectrometry[J],2024.
APA	Tureckova, Veronika,Oklestkova, Jana,Zukauskaite, Asta,Eyer, Ludek,Novak, Ondrej,&Strnad, Miroslav.(2024).A New Abscisic Acid Conjugate, ABA-L-Glutamate, Determined in Different Plant Species by Combined Immunoaffinity Chromatography-Tandem Mass Spectrometry.JOURNAL OF PLANT GROWTH REGULATION.
MLA	Tureckova, Veronika,et al."A New Abscisic Acid Conjugate, ABA-L-Glutamate, Determined in Different Plant Species by Combined Immunoaffinity Chromatography-Tandem Mass Spectrometry".JOURNAL OF PLANT GROWTH REGULATION (2024).