干旱区生态环境知识资源中心(Arid): Establishment of genome-editing system and assembly of a near-complete genome in broomcorn millet

Arid

DOI	10.1111/jipb.13664
	Establishment of genome-editing system and assembly of a near-complete genome in broomcorn millet
	Liu, Yang; Cheng, Zixiang; Chen, Weiyao; Wu, Chuanyin; Chen, Jinfeng; Sui, Yi
通讯作者	Chen, JF
来源期刊	JOURNAL OF INTEGRATIVE PLANT BIOLOGY
ISSN	1672-9072
EISSN	1744-7909
出版年	2024
卷号	66 期号:8 页码:1688-1702
英文摘要	The ancient crop broomcorn millet (Panicum miliaceum L.) is an indispensable orphan crop in semi-arid regions due to its short life cycle and excellent abiotic stress tolerance. These advantages make it an important alternative crop to increase food security and achieve the goal of zero hunger, particularly in light of the uncertainty of global climate change. However, functional genomic and biotechnological research in broomcorn millet has been hampered due to a lack of genetic tools such as transformation and genome-editing techniques. Here, we successfully performed genome editing of broomcorn millet. We identified an elite variety, Hongmi, that produces embryogenic callus and has high shoot regeneration ability in in vitro culture. We established an Agrobacterium tumefaciens-mediated genetic transformation protocol and a clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9-mediated genome-editing system for Hongmi. Using these techniques, we produced herbicide-resistant transgenic plants and edited phytoene desaturase (PmPDS), which is involved in chlorophyll biosynthesis. To facilitate the rapid adoption of Hongmi as a model line for broomcorn millet research, we assembled a near-complete genome sequence of Hongmi and comprehensively annotated its genome. Together, our results open the door to improving broomcorn millet using biotechnology.
英文关键词	broomcorn millet CRISPR/Cas9 editing genome assembly herbicide resistance transformation system
类型	Article
语种	英语
开放获取类型	hybrid
收录类别	SCI-E
WOS记录号	WOS:001214528900001
WOS关键词	WATER-USE EFFICIENCY ; PANICUM-MILIACEUM ; CROP IMPROVEMENT ; ORPHAN CROPS ; ACCURATE ; DROUGHT ; PROGRAM
WOS类目	Biochemistry & Molecular Biology ; Plant Sciences
WOS研究方向	Biochemistry & Molecular Biology ; Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/404636
推荐引用方式 GB/T 7714	Liu, Yang,Cheng, Zixiang,Chen, Weiyao,et al. Establishment of genome-editing system and assembly of a near-complete genome in broomcorn millet[J],2024,66(8):1688-1702.
APA	Liu, Yang,Cheng, Zixiang,Chen, Weiyao,Wu, Chuanyin,Chen, Jinfeng,&Sui, Yi.(2024).Establishment of genome-editing system and assembly of a near-complete genome in broomcorn millet.JOURNAL OF INTEGRATIVE PLANT BIOLOGY,66(8),1688-1702.
MLA	Liu, Yang,et al."Establishment of genome-editing system and assembly of a near-complete genome in broomcorn millet".JOURNAL OF INTEGRATIVE PLANT BIOLOGY 66.8(2024):1688-1702.