干旱区生态环境知识资源中心(Arid): Antivenom potential of the latex of Jatropha mutabilis baill. (Euphorbiaceae) against Tityus stigmurus venom: Evaluating its ability to neutralize toxins and local effects in mice

Arid

DOI	10.1016/j.jep.2024.118642
	Antivenom potential of the latex of Jatropha mutabilis baill. (Euphorbiaceae) against Tityus stigmurus venom: Evaluating its ability to neutralize toxins and local effects in mice
	de Souza, Felipe Santana; de Veras, Bruno Oliveira; Lucena, Lorena de Mendonca; Casoti, Rosana; Martins, Rene Duarte; Ximenes, Rafael Matos
通讯作者	Ximenes, RM
来源期刊	JOURNAL OF ETHNOPHARMACOLOGY
ISSN	0378-8741
EISSN	1872-7573
出版年	2024
卷号	335
英文摘要	Ethnopharmacological relevance: Species of the Jatropha genus (Euphorbiaceae) are used indiscriminately in traditional medicine to treat accidents involving venomous animals. Jatropha mutabilis Baill., popularly known as pinhao-de-seda, o-de-seda, is found in the semi-arid region of Northeastern Brazil. It is widely used as a vermifuge, depurative, laxative, and antivenom. Aim of the study: Obtaining the phytochemical profile of the latex of Jatropha mutabilis (JmLa) and evaluate its acute oral toxicity and inhibitory effects against the venom of the scorpion Tityus stigmurus (TstiV). Materials and methods: The latex of J. mutabilis (JmLa) was obtained through in situ incisions in the stem and characterized using HPLC-ESI-QToF-MS. Acute oral toxicity was investigated in mice. The protein profile of T. stigmurus venom was obtained by electrophoresis. The ability of latex to interact with venom components (TstiV) was assessed using SDS-PAGE, UV-Vis scanning spectrum, and the neutralization of fibrinogenolytic and hyaluronidase activities. Additionally, the latex was evaluated in vivo for its ability to inhibit local edematogenic and nociceptive effects induced by the venom. Results: The phytochemical profile of the latex revealed the presence of 75 compounds, including cyclic peptides, glycosides, phenolic compounds, alkaloids, coumarins, and terpenoids, among others. No signs of acute toxicity were observed at a dose of 2000 mg/kg (p.o.). The latex interacted with the protein profile of TstiV, inhibiting the venom's fibrinogenolytic and hyaluronidase activities by 100%. Additionally, the latex was able to mitigate local envenomation effects, reducing nociception by up to 56.5% and edema by up to 50% compared to the negative control group. Conclusions: The latex of Jatropha mutabilis exhibits a diverse phytochemical composition, containing numerous classes of metabolites. It does not present acute toxic effects in mice and has the ability to inhibit the enzymatic effects of Tityus stigmurus venom in vitro. . Additionally, it reduces nociception and edema in vivo. . These findings corroborate popular reports regarding the antivenom activity of this plant and indicate that the latex has potential for treating scorpionism.
英文关键词	Antivenom Scorpionism Caatinga Nociception Edema
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:001290185700001
WOS关键词	SERRULATUS SCORPION-VENOM ; MEDICINAL-PLANTS ; FUNCTIONAL-CHARACTERIZATION ; METHANOLIC EXTRACT ; CYCLIC PEPTIDE ; SNAKE-VENOM ; HYALURONIDASE ; INHIBITION ; BUTHIDAE ; CAATINGA
WOS类目	Plant Sciences ; Chemistry, Medicinal ; Integrative & Complementary Medicine ; Pharmacology & Pharmacy
WOS研究方向	Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/404503
推荐引用方式 GB/T 7714	de Souza, Felipe Santana,de Veras, Bruno Oliveira,Lucena, Lorena de Mendonca,et al. Antivenom potential of the latex of Jatropha mutabilis baill. (Euphorbiaceae) against Tityus stigmurus venom: Evaluating its ability to neutralize toxins and local effects in mice[J],2024,335.
APA	de Souza, Felipe Santana,de Veras, Bruno Oliveira,Lucena, Lorena de Mendonca,Casoti, Rosana,Martins, Rene Duarte,&Ximenes, Rafael Matos.(2024).Antivenom potential of the latex of Jatropha mutabilis baill. (Euphorbiaceae) against Tityus stigmurus venom: Evaluating its ability to neutralize toxins and local effects in mice.JOURNAL OF ETHNOPHARMACOLOGY,335.
MLA	de Souza, Felipe Santana,et al."Antivenom potential of the latex of Jatropha mutabilis baill. (Euphorbiaceae) against Tityus stigmurus venom: Evaluating its ability to neutralize toxins and local effects in mice".JOURNAL OF ETHNOPHARMACOLOGY 335(2024).