干旱区生态环境知识资源中心(Arid): Method development with high-throughput enhanced matrix removal followed by UHPLC-QqQ-MS/MS for analysis of grape polyphenol metabolites in human urine

Arid

DOI	10.1016/j.jchromb.2024.124189
	Method development with high-throughput enhanced matrix removal followed by UHPLC-QqQ-MS/MS for analysis of grape polyphenol metabolites in human urine
	Lyu, Weiting; Yin, Zhiya; Xie, Lingjun; Pasinetti, Giulio M.; Murrough, James W.; Marchidan, Maxine; Karpman, Elizabeth; Dobbs, Matthew; Ferruzzi, Mario G.; Simon, James E.; Wu, Qingli
通讯作者	Simon, JE ; Wu, QL
来源期刊	JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
ISSN	1570-0232
EISSN	1873-376X
出版年	2024
卷号	1242
英文摘要	Grape and grape derived products contain many bioactive phenolics which have a variety of impacts on health. Following oral ingestion, the phenolic compounds and their metabolites may be detectable in human urine. However, developing a reliable method for the analysis of phenolic compounds in urine is challenging. In this work, we developed and validated a new high-throughput, sensitive and reproducible analytical method for the simultaneous analysis of 31 grape phenolic compounds and metabolites using Oasis PRiME HLB cleanup for sample preparation combined with ultra-performance liquid chromatography with triple quadrupole tandem mass spectrometry (UHPLC-QqQ-MS/MS). Using this new method, the accuracy achieved was 69.3 % similar to 134.9 % (except for six compounds), and the recovery achieved was 52.4 % similar to 134.7 % (except for two very polar compounds). For each of the 31 target analytes, the value of intra-day precision was less than 14.3 %. The value of inter-day precision was slightly higher than intra-day precision, with a range of 0.7 % similar to 19.1 %. We report for the first time on the effect of gender and BMI on the accuracy and recovery of human urine samples, and results from analysis of variance (ANOVA), and principal component analysis (PCA) indicated there was no difference in the value of accuracy and recovery between different gender or BMI (>30) using our purposed cleanup and UHPLC-QqQ-MS/MS method. Overall, this newly developed method could serve as a powerful tool for analyzing grape phenolic compounds and metabolites in human urine samples.
英文关键词	Solid phase extraction Polyphenols Urine metabolites Metabolomics
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:001259952800001
WOS关键词	SPECTROMETRY METHOD ; MASS-SPECTROMETRY ; EXTRACTION ; STABILITY ; AGE
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/404406
推荐引用方式 GB/T 7714	Lyu, Weiting,Yin, Zhiya,Xie, Lingjun,et al. Method development with high-throughput enhanced matrix removal followed by UHPLC-QqQ-MS/MS for analysis of grape polyphenol metabolites in human urine[J],2024,1242.
APA	Lyu, Weiting.,Yin, Zhiya.,Xie, Lingjun.,Pasinetti, Giulio M..,Murrough, James W..,...&Wu, Qingli.(2024).Method development with high-throughput enhanced matrix removal followed by UHPLC-QqQ-MS/MS for analysis of grape polyphenol metabolites in human urine.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1242.
MLA	Lyu, Weiting,et al."Method development with high-throughput enhanced matrix removal followed by UHPLC-QqQ-MS/MS for analysis of grape polyphenol metabolites in human urine".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1242(2024).