干旱区生态环境知识资源中心(Arid): Synthesis of weak cation exchange/C18 bifunctional magnetic polymers for pretreatment and determination of glufosinate and its two metabolites in plasma samples

Arid

DOI	10.1016/j.chroma.2024.464957
	Synthesis of weak cation exchange/C18 bifunctional magnetic polymers for pretreatment and determination of glufosinate and its two metabolites in plasma samples
	Sun, Dier; Gao, Guosheng; Wen, Lili; Xu, Zemin
通讯作者	Xu, ZM
来源期刊	JOURNAL OF CHROMATOGRAPHY A
ISSN	0021-9673
EISSN	1873-3778
出版年	2024
卷号	1725
英文摘要	This study focuses on the purification and detection of glufosinate (GLUF) and its metabolites N-acetyl GLUF and MPP in plasma samples. A Dikma Polyamino HILIC column was used for the effective retention and separation of GLUF and its metabolites, and the innovative addition of a low concentration of ammonium fluoride solution to the mobile phase effectively improved the detection sensitivity of the target analytes. Monodisperse core-shell weak cation exchange (WCX)/C18 bifunctional magnetic polymer composites (Fe3O4@WCX/C18) were prepared in a controllable manner, and their morphology and composition were fully characterized. The Fe3O4@WCX/C18 microspheres were used as a magnetic solid-phase extraction (MSPE) adsorbent for the sample purification and detection of GLUF and its metabolites in plasma samples combined with liquid chromatographytandem mass spectrometry (LC-MS/MS). The purification conditions of Fe3O4@WCX/C18 microspheres for GLUF and its metabolites in spiked plasma samples were optimized to achieve the best MSPE efficiency. The purification mechanisms of the target analytes in plasma samples include electrostatic attraction and hydrophobic interactions. Furthermore, the effect of the molar ratio of the two functional monomers 4-VBA and 1-octadecene in the adsorbent was optimized and it shows that the bifunctional components WCX/C18 have a synergistic effect on the determination of GLUF and its metabolites in plasma samples. In addition, the present study compared the purification performance of the Fe3O4@WCX/C18 microsphere-based MSPE method with that of the commercial Oasis WCX SPE method, and the results showed that the Fe3O4@WCX/C18 microsphere-based MSPE method established in this work had a stronger ability to remove matrix interferences. Under optimal purification conditions, the recoveries of GLUF and its metabolites in plasma were 87.6-111 % with relative standard deviations (RSDs) ranging from 0.2 % to 4.8 %. The limits of detection (LODs, S/N >= 3) and limits of quantification (LOQs, S/ N >= 10) were 0.10-0.18 mu g/L and 0.30-0.54 mu g/L, respectively. The MSPE-LC-MS/MS method developed in this study is fast, simple, accurate and sensitive and can be used to confirm GLUF intoxication based not only on the detection of the GLUF prototype but also on the detection of its two metabolites.
英文关键词	WCX/C 18 bifunctional magnetic polymer composites (Fe 3 O 4 @WCX/C 18 ) HILIC based LC-MS/MS Magnetic solid -phase extraction (MSPE) Glufosinate Metabolites
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:001238186600001
WOS关键词	TANDEM MASS-SPECTROMETRY ; SOLID-PHASE EXTRACTION ; RAPID-DETERMINATION ; AMINOMETHYLPHOSPHONIC ACID ; PRECOLUMN DERIVATIZATION ; GLYPHOSATE ; SERUM ; WATER ; QUANTITATION ; HERBICIDES
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/404404
推荐引用方式 GB/T 7714	Sun, Dier,Gao, Guosheng,Wen, Lili,et al. Synthesis of weak cation exchange/C18 bifunctional magnetic polymers for pretreatment and determination of glufosinate and its two metabolites in plasma samples[J],2024,1725.
APA	Sun, Dier,Gao, Guosheng,Wen, Lili,&Xu, Zemin.(2024).Synthesis of weak cation exchange/C18 bifunctional magnetic polymers for pretreatment and determination of glufosinate and its two metabolites in plasma samples.JOURNAL OF CHROMATOGRAPHY A,1725.
MLA	Sun, Dier,et al."Synthesis of weak cation exchange/C18 bifunctional magnetic polymers for pretreatment and determination of glufosinate and its two metabolites in plasma samples".JOURNAL OF CHROMATOGRAPHY A 1725(2024).