干旱区生态环境知识资源中心(Arid): Simultaneous Determination of D and L Enantiomers of 2-Hydroxyglutarate by UHPLC-MS/MS Method in Human Biological Fluids and its Clinical Application

Arid

DOI	10.1093/chromsci/bmae013
	Simultaneous Determination of D and L Enantiomers of 2-Hydroxyglutarate by UHPLC-MS/MS Method in Human Biological Fluids and its Clinical Application
	Li, Xiaoqing; Bo, Yunhai; Le, Le; Yang, Fen
通讯作者	Yang, F
来源期刊	JOURNAL OF CHROMATOGRAPHIC SCIENCE
ISSN	0021-9665
EISSN	1945-239X
出版年	2024
英文摘要	2-hydroxyglutarate has limited abundance in normal tissues but a high level under certain pathologic conditions. To clarify the diagnostic value of two chiral isomers of 2-hydroxyglutarate in plasma and urine of Chinese cancer patients, an ultra-high performance liquid chromatography-tandem mass spectrometric method was developed for simultaneous quantification of D-/L-2-hydroxyglutarate. The selected D-/L-2-hydroxyglutarate-d5 as internal standards were added to samples before the SPE on Waters Oasis (R) MAX 96-Well plate (30 mu m, 60 mg). A derivatization step with (+)-O,O '-diacetyl-L-tartaric anhydride permitted the chromatography separation of D-/L-2-hydroxyglutarate on an ACQUITY UPLC-HSS T3 column (50 x 2.1 mm, i.d. 1.8 mu m) with acetonitrile and water (containing 0.1% formic acid and 10 mmol ammonium acetate) as the mobile phase. The calibration curves showed good linearity (R >= 0.99) over the concentration ranges of 200-5,000 ng/mL and 500-20,000 ng/mL for analysis of D-/L-2-hydroxyglutarate in plasma and urine samples, respectively. Intra- and inter-run precision were <= 12.33%, and the accuracy was within the range of -10.44 to 13.90%. This method was further successfully applied to clinical sample analysis in isocitrate dehydrogenase 1/2 mutated Chinese cancer patients.
类型	Article ; Early Access
语种	英语
收录类别	SCI-E
WOS记录号	WOS:001193303400001
WOS关键词	TANDEM MASS-SPECTROMETRY ; L-2-HYDROXYGLUTARATE ; MUTATIONS
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/404402
推荐引用方式 GB/T 7714	Li, Xiaoqing,Bo, Yunhai,Le, Le,et al. Simultaneous Determination of D and L Enantiomers of 2-Hydroxyglutarate by UHPLC-MS/MS Method in Human Biological Fluids and its Clinical Application[J],2024.
APA	Li, Xiaoqing,Bo, Yunhai,Le, Le,&Yang, Fen.(2024).Simultaneous Determination of D and L Enantiomers of 2-Hydroxyglutarate by UHPLC-MS/MS Method in Human Biological Fluids and its Clinical Application.JOURNAL OF CHROMATOGRAPHIC SCIENCE.
MLA	Li, Xiaoqing,et al."Simultaneous Determination of D and L Enantiomers of 2-Hydroxyglutarate by UHPLC-MS/MS Method in Human Biological Fluids and its Clinical Application".JOURNAL OF CHROMATOGRAPHIC SCIENCE (2024).