干旱区生态环境知识资源中心(Arid): Quantitation of Venetoclax in Human Plasma by High-Performance Liquid Chromatography with Ultraviolet Detection

Arid

DOI	10.1093/chromsci/bmac080
	Quantitation of Venetoclax in Human Plasma by High-Performance Liquid Chromatography with Ultraviolet Detection
	Fukuda, Natsuki; Kobayashi, Takahiro; Sato, Honami; Akamine, Yumiko; Takahashi, Naoto; Miura, Masatomo
通讯作者	Miura, M
来源期刊	JOURNAL OF CHROMATOGRAPHIC SCIENCE
ISSN	0021-9665
EISSN	1945-239X
出版年	2024
卷号	62 期号:1 页码:58-64
英文摘要	A simple, highly sensitive and specific method based on high-performance liquid chromatography (HPLC) with ultraviolet detection was developed for the measurement of venetoclax concentrations in plasma samples. The chromatographic method employed a mobile phase of acetonitrile: 0.5% KH2PO4 (pH 3.5) (80/20, v/v) on a CAPCELL PAK C18 UG120 column at a flow rate of 0.5 mL/min. The quantitative method was validated based on standards described in Bioanalytical Method Validation: Guidance for Industry published by the US Food and Drug Administration. The separation of venetoclax and the internal standard R051012 was satisfactory, and the chromatograms were free of interfering peaks from the biological matrix. The intra- and inter-day coefficients of variation for venetoclax assays were <12.9%, whereas intra- and inter-day accuracies were within 13.6%. Only 100 mu L of human plasma was required to detect a lower limit of quantification of 10 ng/mL for venetoclax. The recoveries of venetoclax extracted with an Oasis HLB cartridge were between 81 and 85%. The developed HPLC method was successfully applied to the determination of venetoclax concentrations in plasma of acute myeloid leukemia patients taking venetoclax. The degree of drug interactions between venetoclax and CYP3A4 inhibitors can be determined by this HPLC assay.
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000876853600001
WOS关键词	MYELOID-LEUKEMIA PATIENTS ; BCL-2 INHIBITOR ; PHARMACOKINETICS ; QUANTIFICATION ; ITRACONAZOLE ; METABOLISM
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/404401
推荐引用方式 GB/T 7714	Fukuda, Natsuki,Kobayashi, Takahiro,Sato, Honami,et al. Quantitation of Venetoclax in Human Plasma by High-Performance Liquid Chromatography with Ultraviolet Detection[J],2024,62(1):58-64.
APA	Fukuda, Natsuki,Kobayashi, Takahiro,Sato, Honami,Akamine, Yumiko,Takahashi, Naoto,&Miura, Masatomo.(2024).Quantitation of Venetoclax in Human Plasma by High-Performance Liquid Chromatography with Ultraviolet Detection.JOURNAL OF CHROMATOGRAPHIC SCIENCE,62(1),58-64.
MLA	Fukuda, Natsuki,et al."Quantitation of Venetoclax in Human Plasma by High-Performance Liquid Chromatography with Ultraviolet Detection".JOURNAL OF CHROMATOGRAPHIC SCIENCE 62.1(2024):58-64.