干旱区生态环境知识资源中心(Arid): Bioprospecting of endophytic fungi from semidesert candelilla (Euphorbia antisyphilitica Zucc): Potential for extracellular enzyme production

Arid

DOI	10.1002/jobm.202400049
	Bioprospecting of endophytic fungi from semidesert candelilla (Euphorbia antisyphilitica Zucc): Potential for extracellular enzyme production
	Nunez-Garcia, Itzel C.; Martinez-avila, Guillermo Cristian G.; Gonzalez-Herrera, Silvia M.; Tafolla-Arellano, Julio C.; Rutiaga-Quinones, O. Miriam
通讯作者	Rutiaga-Quiñones, OM
来源期刊	JOURNAL OF BASIC MICROBIOLOGY
ISSN	0233-111X
EISSN	1521-4028
出版年	2024
卷号	64 期号:7
英文摘要	Endophytic microbial communities colonize plants growing under various abiotic stress conditions. Candelilla (Euphorbia antisyphilitica Zucc.) is a shrub that develops functionally in arid and semi-arid zones of Mexico; these conditions generate an association between the plant and the microorganisms, contributing to the production of enzymes as a defense mechanism for resistance to abiotic stress. The objective of this research was to isolate and identify endophyte fungi of candelilla and bioprospection of these endophytic fungi for enzyme production using candelilla by-products. Fungi were isolated and identified using ITS1/ITS4 sequencing. Their potency index (PI) was evaluated in producing endoglucanase, xylanase, amylase, and laccase. Fermentation was carried out at 30 degrees C for 8 days at 200 rpm, with measurements every 2 days, using candelilla by-products as substrate. All fungi exhibited higher cellulase, amylase, and laccase activities on the 2nd, 6th, and 8th day of fermentation, respectively, of fermentation. The fungus Aspergillus niger ITD-IN4.1 showed the highest amylase activity (246.84 U/mg), the genus Neurospora showed the highest cellulase activity, reaching up to 13.45 FPU/mg, and the strain Neurospora sp. ITD-IN5.2 showed the highest laccase activity (3.46 U/mg). This work provides the first report on the endophytic diversity of E. antisyphilitica and its potential role in enzyme production.
英文关键词	Aspergillus by-products cellulases laccase molecular identification
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:001215263300001
WOS关键词	ASPERGILLUS-FLAVUS ; LACCASE PRODUCTION ; SOLID-STATE ; IDENTIFICATION ; OPTIMIZATION ; CELLULASE ; STRAINS ; WASTE ; NIGER
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/404391
推荐引用方式 GB/T 7714	Nunez-Garcia, Itzel C.,Martinez-avila, Guillermo Cristian G.,Gonzalez-Herrera, Silvia M.,et al. Bioprospecting of endophytic fungi from semidesert candelilla (Euphorbia antisyphilitica Zucc): Potential for extracellular enzyme production[J],2024,64(7).
APA	Nunez-Garcia, Itzel C.,Martinez-avila, Guillermo Cristian G.,Gonzalez-Herrera, Silvia M.,Tafolla-Arellano, Julio C.,&Rutiaga-Quinones, O. Miriam.(2024).Bioprospecting of endophytic fungi from semidesert candelilla (Euphorbia antisyphilitica Zucc): Potential for extracellular enzyme production.JOURNAL OF BASIC MICROBIOLOGY,64(7).
MLA	Nunez-Garcia, Itzel C.,et al."Bioprospecting of endophytic fungi from semidesert candelilla (Euphorbia antisyphilitica Zucc): Potential for extracellular enzyme production".JOURNAL OF BASIC MICROBIOLOGY 64.7(2024).