干旱区生态环境知识资源中心(Arid): Molecular Identification of Genetic Diversity and Population Structure in Moroccan Male Date Palm (Phoenix dactylifera L.) Using Inter-Simple Sequence Repeat, Direct Amplification of Minisatellite DNA, and Simple Sequence Repeat Markers

Arid

DOI	10.3390/horticulturae10050508
	Molecular Identification of Genetic Diversity and Population Structure in Moroccan Male Date Palm (Phoenix dactylifera L.) Using Inter-Simple Sequence Repeat, Direct Amplification of Minisatellite DNA, and Simple Sequence Repeat Markers
	Ibrahimi, Maha; Brhadda, Najiba; Ziri, Rabea; Fokar, Mohamed; Amghar, Ilham; Gaboun, Fatima; Habach, Aicha; Meziani, Reda; Elfadile, Jamal; Abdelwahd, Rabha; Diria, Ghizlane
通讯作者	Diria, G
来源期刊	HORTICULTURAE
EISSN	2311-7524
出版年	2024
卷号	10 期号:5
英文摘要	Understanding genetic diversity and population structure plays a vital role in the efficient use of available material in plant-breeding programs and in germplasm conservation strategies. In the present study, we aim to evaluate the genetic variations and population structure of male date palms from Morocco. The genetic diversity of 100 date palm (Phoenix dactylifera L.) genotypes was investigated using the performance of three types of molecular markers: inter-simple sequence repeats (ISSRs), direct amplification of minisatellite DNA (DAMD), and simple sequence repeats (SSRs). On the basis of their polymorphic information content (PIC) (ISSRs = 0.38; DAMD = 0.4; SSRs = 0.33), effective multiplex ratio (EMR) (ISSRs = 27.34; DAMD = 52.31; SSRs = 22.20), Resolving power Rp (ISSR = 13.81; DAMD = 28.73; SSR = 14.6), and marker index (MI) (ISSRs = 9.22; DAMD = 20.23; SSRs = 7.54) values, all markers used in our study are considered informative markers. Among them, DAMD markers demonstrated slightly higher informativeness compared to ISSR and SSR markers. A total of 216, 438, and 248 bands were, respectively, detected using ISSRs, DAMD, and SSRs, with 95%, 98% and 94% of polymorphism, respectively. The AMOVA results revealed considerable diversity within date palms. The PCOa results showed that males of Tinghir and Errachidia were regrouped into the same cluster, while males of Goulmima were separated into another group. A cluster and structure analysis separated the studied genotypes into three groups. One group comprises genotypes of males from Zagora with some female varieties scattered in this group. The second group includes male genotypes from Goulmima along with accessions of female and male varieties. The third group contains males of Errachidia, Tata and Tinghir populations. The cluster and structure analysis separated the studied genotypes according to their origin.
英文关键词	genetic diversity Phoenix dactylifera L. ISSR DAMD SSR structure
类型	Article
语种	英语
开放获取类型	gold
收录类别	SCI-E
WOS记录号	WOS:001233525100001
WOS关键词	MICROSATELLITE MARKERS ; SSR ; POLYMORPHISM ; CULTIVARS ; GERMPLASM ; RAPD ; VARIETIES ; GENOTYPES ; SYSTEM ; OASES
WOS类目	Horticulture
WOS研究方向	Agriculture
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/404065
推荐引用方式 GB/T 7714	Ibrahimi, Maha,Brhadda, Najiba,Ziri, Rabea,et al. Molecular Identification of Genetic Diversity and Population Structure in Moroccan Male Date Palm (Phoenix dactylifera L.) Using Inter-Simple Sequence Repeat, Direct Amplification of Minisatellite DNA, and Simple Sequence Repeat Markers[J],2024,10(5).
APA	Ibrahimi, Maha.,Brhadda, Najiba.,Ziri, Rabea.,Fokar, Mohamed.,Amghar, Ilham.,...&Diria, Ghizlane.(2024).Molecular Identification of Genetic Diversity and Population Structure in Moroccan Male Date Palm (Phoenix dactylifera L.) Using Inter-Simple Sequence Repeat, Direct Amplification of Minisatellite DNA, and Simple Sequence Repeat Markers.HORTICULTURAE,10(5).
MLA	Ibrahimi, Maha,et al."Molecular Identification of Genetic Diversity and Population Structure in Moroccan Male Date Palm (Phoenix dactylifera L.) Using Inter-Simple Sequence Repeat, Direct Amplification of Minisatellite DNA, and Simple Sequence Repeat Markers".HORTICULTURAE 10.5(2024).