干旱区生态环境知识资源中心(Arid): Aeluropus littoralis stress-associated protein promotes water deficit resilience in engineered durum wheat

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DOI	10.1016/j.heliyon.2024.e30933
	Aeluropus littoralis stress-associated protein promotes water deficit resilience in engineered durum wheat
	Romdhane, Walid Ben; Al-Ashkar, Ibrahim; Ibrahim, Abdullah; Sallam, Mohammed; Al-Doss, Abdullah; Hassairi, Afif
通讯作者	Romdhane, WB ; Al-Ashkar, I ; Hassairi, A
来源期刊	HELIYON
EISSN	2405-8440
出版年	2024
卷号	10 期号:10
英文摘要	Global climate change-related water deficit negatively affect the growth, development and yield performance of multiple cereal crops, including durum wheat. Therefore, the improvement of water-deficit stress tolerance in durum wheat varieties in arid and semiarid areas has become imperative for food security. Herein, we evaluated the water deficiency resilience potential of two marker-free transgenic durum wheat lines (AlSAP-lines: K9.3 and K21.3) under well-watered and water-deficit stress conditions at both physiological and agronomic levels. These two lines overexpressed the AlSAP gene, isolated from the halophyte grass Aeluropus littoralis, encoding a stress-associated zinc finger protein containing the A20/AN1 domains. Under well-watered conditions, the wild-type (WT) and both AlSAP-lines displayed comparable performance concerning all the evaluated parameters. Ectopic transgene expression exerted no adverse effects on growth and yield performance of the durum wheat plants. Under water-deficit conditions, no significant differences in the plant height, leaf number, spike length, and spikelet number were observed between AlSAP-lines and WT plants. However, compared to WT, the AlSAP-lines exhibited greater dry matter production, greater flag leaf area, improved net photosynthetic rate, stomatal conductance, and water use efficiency. Notably, the AlSAP-lines displayed 25 % higher grain yield (GY) than the WT plants under water-deficit conditions. The RT-qPCR-based selected stress-related gene (TdDREB1, TdLEA, TdAPX1, and TdBlt101-2) expression analyses indicated stress-related genes enhancement in AlSAP-durum wheat plants under both well-watered and water-deficit conditions, potentially related to the water-deficit resilience. Collectively, our findings support that the ectopic AlSAP expression in durum wheat lines enhances water-deficit resilience ability, thereby potentially compensate for the GY loss in arid and semi-arid regions.
英文关键词	Stress -associated protein Aeluropus littoralis Marker-free genetically engineered durum wheat Water deficiency Multivariate analysis
类型	Article
语种	英语
开放获取类型	gold
收录类别	SCI-E
WOS记录号	WOS:001240550000001
WOS关键词	SAP GENE FAMILY ; CANOPY TEMPERATURE ; GRAIN-YIELD ; STOMATAL CONDUCTANCE ; TRANSGENIC TOBACCO ; CONFERS TOLERANCE ; DROUGHT ; SALT ; RICE ; COMPONENTS
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/404029
推荐引用方式 GB/T 7714	Romdhane, Walid Ben,Al-Ashkar, Ibrahim,Ibrahim, Abdullah,et al. Aeluropus littoralis stress-associated protein promotes water deficit resilience in engineered durum wheat[J],2024,10(10).
APA	Romdhane, Walid Ben,Al-Ashkar, Ibrahim,Ibrahim, Abdullah,Sallam, Mohammed,Al-Doss, Abdullah,&Hassairi, Afif.(2024).Aeluropus littoralis stress-associated protein promotes water deficit resilience in engineered durum wheat.HELIYON,10(10).
MLA	Romdhane, Walid Ben,et al."Aeluropus littoralis stress-associated protein promotes water deficit resilience in engineered durum wheat".HELIYON 10.10(2024).