干旱区生态环境知识资源中心(Arid): Epidemiology and phylogeny of Haemonchus contortus through internal transcribed spacer 2 gene in small ruminants

Arid

DOI	10.3389/fvets.2024.1380203
	Epidemiology and phylogeny of Haemonchus contortus through internal transcribed spacer 2 gene in small ruminants
	Ahmad, Nisar; Khan, Saeed A.; Majid, Hafiz A.; Ali, Rehman; Ullah, Riaz; Bari, Ahmed; Akbar, Noor Ul; Majid, Abdul
通讯作者	Ahmad, N
来源期刊	FRONTIERS IN VETERINARY SCIENCE
EISSN	2297-1769
出版年	2024
卷号	11
英文摘要	Introduction: Haemonchus contortus (H. contortus) is a blood-feeding nematode causing infectious disease haemonchosis in small ruminants of tropical and subtropical regions around the world. This study aimed to explore the prevalence and phylogeny of H. contortus in small ruminants using the internal transcribed spacer-2 (ITS-2) gene. In addition, a comprehensive review of the available literature on the status of H. contortus in Pakistan was conducted. Methods: Fecal samples were collected from sheep and goats (n = 180). Microscopically positive samples were subjected to DNA extraction followed by PCR using species-specific primers. Results: The overall prevalence of H. contortus was 25.55% in small ruminants. The prevalence of H. contortus was significantly associated with months and area. The highest occurrence of haemonchosis was documented in July (38.70%), whereas the lowest occurred in December (11.11%), with significant difference. The prevalence was highest in the Ghamkol camp (29.4%) and lowest in the arid zone of the Small Ruminant Research Institute (17.5%) (p = 0.01). The results of the systematic review revealed the highest prevalence of haemonchosis (34.4%) in Khyber Pakhtunkhwa (p = 0.001). Discussion: Phylogenetic analysis revealed a close relationship between H. contortus and isolates from Asia (China, India, Iran, Bangladesh, Malaysia, and Mongolia) and European countries (Italy and the United Kingdom). It has been concluded that H. contortus is prevalent in small ruminants of Kohat district and all over Pakistan, which could be a potential threat to food-producing animals, farmers, dairy, and the meat industry. Phylogenetic analysis indicates that H. contortus isolates share close phylogenetic relationships with species from Asia and Europe.
英文关键词	Haemonchus contortus prevalence small ruminants ITS-2 PCR phylogenetic analysis epidemiology phylogeny
类型	Article
语种	英语
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:001206380300001
WOS关键词	GASTROINTESTINAL PARASITES ; DOMESTIC RUMINANTS ; POPULATION-STRUCTURE ; SOUTHERN PUNJAB ; INFECTION-RATE ; SHEEP ; PREVALENCE ; GOATS ; DISTRICT ; NEMATODES
WOS类目	Veterinary Sciences
WOS研究方向	Veterinary Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/403903
推荐引用方式 GB/T 7714	Ahmad, Nisar,Khan, Saeed A.,Majid, Hafiz A.,et al. Epidemiology and phylogeny of Haemonchus contortus through internal transcribed spacer 2 gene in small ruminants[J],2024,11.
APA	Ahmad, Nisar.,Khan, Saeed A..,Majid, Hafiz A..,Ali, Rehman.,Ullah, Riaz.,...&Majid, Abdul.(2024).Epidemiology and phylogeny of Haemonchus contortus through internal transcribed spacer 2 gene in small ruminants.FRONTIERS IN VETERINARY SCIENCE,11.
MLA	Ahmad, Nisar,et al."Epidemiology and phylogeny of Haemonchus contortus through internal transcribed spacer 2 gene in small ruminants".FRONTIERS IN VETERINARY SCIENCE 11(2024).