干旱区生态环境知识资源中心(Arid): Flow cytometric analysis of immune cell populations in the bronchial and mesenteric lymph nodes of the dromedary camel

Arid

DOI	10.3389/fvets.2024.1365319
	Flow cytometric analysis of immune cell populations in the bronchial and mesenteric lymph nodes of the dromedary camel
	Hussen, Jamal; Althagafi, Hind; Al-Sukruwah, Mohammed Ali; Falemban, Baraa; Abdul Manap, Aimi Syamima
通讯作者	Hussen, J
来源期刊	FRONTIERS IN VETERINARY SCIENCE
EISSN	2297-1769
出版年	2024
卷号	11
英文摘要	Dromedary camel is an important livestock species with special economic value in arid and semi-arid regions of the world. Given the limited data on detailed immune cell composition and cell marker expression in the dromedary camel lymph node tissue, the present study was undertaken to investigate the immune cell composition of bronchial and mesenteric lymph nodes from healthy dromedary camels using flow cytometry. In this study, we applied flow cytometry and multicolor immuno-fluorescence to phenotype the main populations of immune cells in the bronchial and mesenteric camel lymph nodes and compared them with separated peripheral blood mononuclear cells and granulocytes. We used antibodies to detect several cell surface molecules associated with camel T cells (CD4, WC1), B cells (MHCII, BAQ44A), monocytes/macrophages (CD172a, CD14, CD163), in addition to the pan-leukocyte marker CD45 and the cell adhesion molecules CD44 and CD18. Compared to blood mononuclear cells, camel lymph node cells contained a higher percentage of lymphoid cells with only a minor fraction of myeloid cells. In addition, the lower expression of CD44 and CD18 on lymph node lymphocytes compared to lymphocytes from peripheral blood indicates higher frequency of na & iuml;ve lymphocytes in the lymph nodes. The frequency of CD4+ T cells, B cells and gamma delta T cells within camel lymph node lymphocytes compared to blood indicates a similar tissue distribution pattern of lymphocyte subsets in camel and bovine and supports previous reports on the similarity between the camel immune system and the immune system of other ruminants. Lymph node neutrophils were identified as CD45++ CD172a++, CD14+, MHCIIlow, BAQ44A+, CD44++, CD18++ cells. In conclusion, the present study is describing the employment of flow cytometric single-cell analysis and immunostaining for the analysis of the immune cell composition in the camel lymph node.
英文关键词	camel immune system lymph node B cells T cells flow cytometry
类型	Article
语种	英语
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:001221589900001
WOS关键词	CD4(+) T-CELLS ; MHC CLASS-II ; PERIPHERAL-BLOOD ; MONOCLONAL-ANTIBODIES ; EXPRESSION ; ORGANS ; SUBPOPULATIONS ; PROLIFERATION ; PHENOTYPE ; BACTERIAL
WOS类目	Veterinary Sciences
WOS研究方向	Veterinary Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/403902
推荐引用方式 GB/T 7714	Hussen, Jamal,Althagafi, Hind,Al-Sukruwah, Mohammed Ali,et al. Flow cytometric analysis of immune cell populations in the bronchial and mesenteric lymph nodes of the dromedary camel[J],2024,11.
APA	Hussen, Jamal,Althagafi, Hind,Al-Sukruwah, Mohammed Ali,Falemban, Baraa,&Abdul Manap, Aimi Syamima.(2024).Flow cytometric analysis of immune cell populations in the bronchial and mesenteric lymph nodes of the dromedary camel.FRONTIERS IN VETERINARY SCIENCE,11.
MLA	Hussen, Jamal,et al."Flow cytometric analysis of immune cell populations in the bronchial and mesenteric lymph nodes of the dromedary camel".FRONTIERS IN VETERINARY SCIENCE 11(2024).