干旱区生态环境知识资源中心(Arid): Drought stress tolerance and metabolomics of Medicago sativa induced by Bacillus amyloliquefaciens DGL1

Arid

DOI	10.3389/fpls.2024.1378707
	Drought stress tolerance and metabolomics of Medicago sativa induced by Bacillus amyloliquefaciens DGL1
	Yang, Xue; Xie, Yongli; Qiao, Youming; Chang, Feifei; Wang, Tian; Li, Junxi; Wu, Lingling; Li, Chen; Gao, Ying
通讯作者	Xie, YL
来源期刊	FRONTIERS IN PLANT SCIENCE
ISSN	1664-462X
出版年	2024
卷号	15
英文摘要	Introduction This study used Bacillus amyloliquefaciens DGL1 isolated from the arid sandy land of the Qinghai-Tibetan Plateau as the research strain and investigated the effects of DGL1 on the biomass, physiology, and metabolites of Medicago sativa under different intensities of drought stress to provide a high-quality bacterial source and a theoretical basis for the research and development of biological fertilizer suitable for arid areas. Methods The exopolysaccharides (EPS), 1-Aminocyclopropane-1-carboxylate deaminase (ACC), and phosphorus solubilizing capacity of DGL1 were determined. The effects of a DGL1 suspension on alfalfa biomass, physiological indexes, degree of peroxidation of cell membranes, and activity of antioxidant enzymes were determined after irrigating roots under drought stress. The effects on soil physicochemical properties were also evaluated, and metabolomics analysis was performed to explore the effect of DGL1 on the metabolites of alfalfa under drought stress. Results Strain DGL1 produced extracellular polysaccharide EPS and ACC deaminase and was capable of phosphorus solubilization. Treatment with DGL1 increased the biomass of alfalfa under different degrees of drought stress, significantly increased the activities of alfalfa antioxidant enzymes Super Oxide Dismutase (SOD), Peroxidase (POD), and catalase (CAT), reduced the content of MDA and H2O2, and increased the content of quick-acting phosphorus, quick-acting potassium, ammonium nitrogen, and nitrate nitrogen in the soil, thus improving soil fertility. Through metabolomics analysis, DGL1 was shown to affect amino acid metabolic pathways, such as arginine, leucine, glutamate, and tyrosine, as well as the levels of energy-providing polysaccharides and lipids, in alfalfa under 15% PEG-6000 drought stress, enhancing alfalfa's capacity to resist drought stress. Discussion Strain DGL1 enhances the drought suitability of alfalfa and has the potential for dryland development as a biological agent.
英文关键词	Bacillus amylolyticus alfalfa drought stress antioxidant enzymes growth promotion metabolomics
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:001231002800001
WOS关键词	GROWTH ; SUBTILIS ; YIELD ; PHOSPHORUS ; POTASSIUM
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/403872
推荐引用方式 GB/T 7714	Yang, Xue,Xie, Yongli,Qiao, Youming,et al. Drought stress tolerance and metabolomics of Medicago sativa induced by Bacillus amyloliquefaciens DGL1[J],2024,15.
APA	Yang, Xue.,Xie, Yongli.,Qiao, Youming.,Chang, Feifei.,Wang, Tian.,...&Gao, Ying.(2024).Drought stress tolerance and metabolomics of Medicago sativa induced by Bacillus amyloliquefaciens DGL1.FRONTIERS IN PLANT SCIENCE,15.
MLA	Yang, Xue,et al."Drought stress tolerance and metabolomics of Medicago sativa induced by Bacillus amyloliquefaciens DGL1".FRONTIERS IN PLANT SCIENCE 15(2024).