干旱区生态环境知识资源中心(Arid): Evolutionary Characterization of tubulin Gene Family in the Desert Biomass Willow (Salix psammophila) and Expression of the β-tubulin Gene SpsTUB10 during Different Stresses

Arid

DOI	10.3390/f15040696
	Evolutionary Characterization of tubulin Gene Family in the Desert Biomass Willow (Salix psammophila) and Expression of the β-tubulin Gene SpsTUB10 during Different Stresses
	He, Yujiao; Fan, Lijiao; Wang, Ruiping; Han, Shengli; Sun, Guirong; Yu, Fengqiang; Yang, Qi; Yang, Haifeng; Zhang, Guosheng
通讯作者	Yang, HF ; Zhang, GS
来源期刊	FORESTS
EISSN	1999-4907
出版年	2024
卷号	15 期号:4
英文摘要	Microtubules, polymerized from alpha-tubulin (TUA) and beta-tubulin (TUB) monomers, play a pivotal role in shaping plant morphogenesis according to developmental and environmental cues. Salix psammophila C. Wang & C. Y. Yang is an important shrub plant in sand-fixing afforestation in arid regions, with three significantly distinct plant types shaped under various environments, namely, upright, intermediate, and scattered types. However, how tubulin genes respond to the developmental and environmental signs in S. psammophila has been far less studied. Here, based on RNA-seq, Sanger sequencing, and real-time PCR (RT-PCR) data, we analyzed the phylogeny of tubulins and their expression profiles in S. psammophila among the three plant types. Furthermore, we analyzed the genetic structure and expression pattern of SpsTUB10 in S. psammophila under various abiotic stress treatments. In total, we identified 26 SpsTubulin genes in S. psammophila. The homologous alignment and phylogenetic analysis revealed that these SpsTubulin genes can be classified into two groups, corresponding to the TUA and TUB genes. The expression profiles of these SpsTubulin genes in various organs showed that most SpsTubulin genes were mainly expressed in the root. SpsTUB10 is a member of the TUB IIa group, consisting of two intros and three exons. The SpsTUB10 protein contains a typical GTPase domain and a C-terminal domain, with alpha-helix and random coil dominant in the secondary and tertiary structures. The RT-PCR results of SpsTUB10 showed an extremely significant difference in expression levels among the root and stem-developing organs between the upright and scattered types, and the transcript level of SpsTUB10 had a significantly negative correlation with the crown-height ratio. Under different treatments, we found that cold, osmotic stress, and short daylight could significantly increase SpsTUB10 expression levels compared to those in the controls, thereby supporting the positive role of SpsTUB10 in stress-induced responses. These results will provide evidence for the SpsTubulin genes' response to the developmental and environmental cues in S. psammophila.
英文关键词	Salix psammophila tubulin SpsTUB10 evolutionary analysis expression analysis abiotic stress
类型	Article
语种	英语
开放获取类型	gold
收录类别	SCI-E
WOS记录号	WOS:001220474700001
WOS关键词	MICROTUBULES ; ALPHA ; TRANSCRIPTS ; EXPANSION ; PROTEIN
WOS类目	Forestry
WOS研究方向	Forestry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/403787
推荐引用方式 GB/T 7714	He, Yujiao,Fan, Lijiao,Wang, Ruiping,et al. Evolutionary Characterization of tubulin Gene Family in the Desert Biomass Willow (Salix psammophila) and Expression of the β-tubulin Gene SpsTUB10 during Different Stresses[J],2024,15(4).
APA	He, Yujiao.,Fan, Lijiao.,Wang, Ruiping.,Han, Shengli.,Sun, Guirong.,...&Zhang, Guosheng.(2024).Evolutionary Characterization of tubulin Gene Family in the Desert Biomass Willow (Salix psammophila) and Expression of the β-tubulin Gene SpsTUB10 during Different Stresses.FORESTS,15(4).
MLA	He, Yujiao,et al."Evolutionary Characterization of tubulin Gene Family in the Desert Biomass Willow (Salix psammophila) and Expression of the β-tubulin Gene SpsTUB10 during Different Stresses".FORESTS 15.4(2024).