干旱区生态环境知识资源中心(Arid): Development and performance evaluation of a microbiological method for screening and LC-MS/MS for conformation of sulfonamides in animal-derived foods

Arid

DOI	10.1080/19440049.2024.2368903
	Development and performance evaluation of a microbiological method for screening and LC-MS/MS for conformation of sulfonamides in animal-derived foods
	Kanda, Maki; Sekimura, Kotaro; Yoshikawa, Souichi; Hayashi, Hiroshi; Ohba, Yumi; Koike, Hiroshi; Matsushima, Yoko; Hayashi, Momoka; Nagano, Chieko; Sasamoto, Takeo
通讯作者	Kanda, M
来源期刊	FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
ISSN	1944-0049
EISSN	1944-0057
出版年	2024
卷号	41 期号:8 页码:900-913
英文摘要	This study developed a highly sensitive microbiological method utilizing a novel microtiter plate to screen 10 sulfonamides in chicken muscles, eggs, and prawns. This plate was fabricated from agar incorporating trimethoprim and spread with Bacillus megaterium. After residue detection by bioassay, the same test solutions were analyzed by LC-MS/MS for accurate identification and quantification. It also proved eco-friendly compared to using other quantitative methods. The residual drugs were extracted with McIlvaine buffer and purified using an Oasis (R) MCX cartridge. A triethylamine/methanol/water (0.5:75:24.5, v/v/v) mixture was used as the eluate. The obtained LOD values of the bioassay ranged from 5 to 25 mu g kg-(1) allowing the detection of the target drugs at the MRLs established in Japan. Adhering to ISO/IEC 17025 standards, the performance of the bioassay was evaluated. Based on the inhibition zone size in bioassay results, quality control yielded a Z score within +/- 2, indicating reasonable control over the screening process. Proficiency testing of a chicken muscle sample spiked with sulfadimidine demonstrated the inhibition zone detection of the bioassay and quantified value alignment of LC-MS/MS with reference values. In a surveillance study of 91 samples, sulfamethoxazole was detected in one prawn sample.
英文关键词	Microbiological screening method Bacillus megaterium sulfonamide animal-derived food proficiency testing
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:001262772600001
WOS关键词	MULTI-RESIDUE DETERMINATION ; TANDEM MASS-SPECTROMETRY ; VETERINARY DRUG RESIDUES ; LIQUID-CHROMATOGRAPHY ; ANTIBIOTIC-RESIDUES ; SAMPLE PREPARATION ; VALIDATION ; PRODUCTS ; MEAT ; PHASE
WOS类目	Chemistry, Applied ; Food Science & Technology ; Toxicology
WOS研究方向	Chemistry ; Food Science & Technology ; Toxicology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/403747
推荐引用方式 GB/T 7714	Kanda, Maki,Sekimura, Kotaro,Yoshikawa, Souichi,et al. Development and performance evaluation of a microbiological method for screening and LC-MS/MS for conformation of sulfonamides in animal-derived foods[J],2024,41(8):900-913.
APA	Kanda, Maki.,Sekimura, Kotaro.,Yoshikawa, Souichi.,Hayashi, Hiroshi.,Ohba, Yumi.,...&Sasamoto, Takeo.(2024).Development and performance evaluation of a microbiological method for screening and LC-MS/MS for conformation of sulfonamides in animal-derived foods.FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT,41(8),900-913.
MLA	Kanda, Maki,et al."Development and performance evaluation of a microbiological method for screening and LC-MS/MS for conformation of sulfonamides in animal-derived foods".FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT 41.8(2024):900-913.