干旱区生态环境知识资源中心(Arid): Simultaneous determination of five carbapenems, highly polar antibiotics, in milk by LC-MS/MS

Arid

DOI	10.1080/19440049.2023.2300338
	Simultaneous determination of five carbapenems, highly polar antibiotics, in milk by LC-MS/MS
	Ohba, Yumi; Hayashi, Hiroshi; Kanda, Maki; Nagano, Chieko; Yoshikawa, Souichi; Nakajima, Takayuki; Matsushima, Yoko; Koike, Hiroshi; Hayashi, Momoka; Yokoyama, Tomoko; Sasamoto, Takeo
通讯作者	Ohba, Y
来源期刊	FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
ISSN	1944-0049
EISSN	1944-0057
出版年	2024
卷号	41 期号:2 页码:151-161
英文摘要	The simultaneous determination of five carbapenems (biapenem, doripenem, ertapenem, imipenem, and meropenem) in raw and pasteurised bovine milk samples using LC-MS/MS was achieved and validated. Chromatographic separation was conducted on an InertSustain (R) AQ-C18 column using 0.1% formic acid in water and acetonitrile as the mobile phase. Target compounds were extracted using acetonitrile/water (20:80, v/v). After the removal of lipids with acetonitrile-saturated hexane, the dissolved protein was denatured with acetic acid. A portion of the supernatant was passed through an Oasis (R) PRiME HLB cartridge to remove the matrix. This novel method was validated in accordance with the Japanese validation guidelines and exhibited good trueness, ranging from 86.3% to 96.2%, using matrix-matched calibration curves. The relative standard deviation of repeatability ranged from 1.0% to 6.3%, and that of within-laboratory reproducibility ranged from 1.6% to 7.1%. The limit of quantification was 1.0 mu g kg-1 for all analytes. None of the 60 milk samples commercially available in Tokyo contained any analytes. This novel method exhibited high-quality performance and can easily be implemented for the routine monitoring of carbapenems, which are highly polar antibiotics in milk.
英文关键词	Carbapenems highly polar antibiotics LC-MS/MS raw bovine milk pasteurised bovine milk
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:001147074500001
WOS关键词	BETA-LACTAM ANTIBIOTICS ; BOVINE-MILK ; MEROPENEM ; RESIDUES
WOS类目	Chemistry, Applied ; Food Science & Technology ; Toxicology
WOS研究方向	Chemistry ; Food Science & Technology ; Toxicology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/403746
推荐引用方式 GB/T 7714	Ohba, Yumi,Hayashi, Hiroshi,Kanda, Maki,et al. Simultaneous determination of five carbapenems, highly polar antibiotics, in milk by LC-MS/MS[J],2024,41(2):151-161.
APA	Ohba, Yumi.,Hayashi, Hiroshi.,Kanda, Maki.,Nagano, Chieko.,Yoshikawa, Souichi.,...&Sasamoto, Takeo.(2024).Simultaneous determination of five carbapenems, highly polar antibiotics, in milk by LC-MS/MS.FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT,41(2),151-161.
MLA	Ohba, Yumi,et al."Simultaneous determination of five carbapenems, highly polar antibiotics, in milk by LC-MS/MS".FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT 41.2(2024):151-161.