干旱区生态环境知识资源中心(Arid): Diversity and spectrum of pathogenicity of fungi associated with wilt disease of alfalfa in southern Tunisian oases

Arid

DOI	10.1007/s10658-024-02871-8
	Diversity and spectrum of pathogenicity of fungi associated with wilt disease of alfalfa in southern Tunisian oases
	Ben Alaya, Amani; Khiari, Bilel; Ben Slimene, Imen; Chaouachi, Manel; Badri, Mounawer; Gentzbittel, Laurent; Ben, Cecile; Djebali, Naceur
通讯作者	Djébali, N
来源期刊	EUROPEAN JOURNAL OF PLANT PATHOLOGY
ISSN	0929-1873
EISSN	1573-8469
出版年	2024
卷号	169 期号:4 页码:799-823
英文摘要	Wilt disease affects alfalfa plants (Medicago sativa) by disrupting their vascular tissues which limits water and nutrient uptakes leading to yield reduction. Field surveys conducted in 2017 in the oases of Southern Tunisia showed serious wilting symptoms in alfalfa, associated with leaves yellowing and root rotting. This study aims to identify the causal agents of alfalfa wilting disease, along with their cultural biodiversity and ecology of infestation. Wilted plants from six oases were sampled which led to the isolation of 79 fungal strains from roots, collars, and stems. The inoculation of the fungal strains on Medicago sativa (cv. El-Hamma) and Medicago truncatula (accessions A17 and F83005.5) induced different reactions in plants depending on the inoculated Medicago species and accession. The majority of the strains did not affect plant growth (59-86%), the second group enhanced plant growth (6-16%), and the third group induced disease symptoms associated with a reduction in seedling growth (8-24%). The molecular identification showed that 73.42% of the fungal strains belong to the genus Fusarium, including the species complexes of F. oxysporum, F. solani, F. fujikuroi, F. incarnatum-equiseti and, F. chlamydosporum; 10.13% to Alternaria; 5.06% to Rhizoctonia; and 1.27% to Aaosphaeria. The pathogenicity tests on date palm seedlings (cv. Deglet Nour) showed that strains affiliated with the genera Rhizoctonia sp. and Fusarium fujikuroi species complex reduced the growth of plants, suggesting that alfalfa constitutes an alternate host for these pathogens. Alfalfa wilting disease in Southern Tunisian oases is caused by a complex of pathogens rather than a single agent, as it is caused by a consortium of mutually interacting Fusarium species complexes (F. oxysporum, F. solani, F. fujikuroi and, F. incarnatum-equiseti), Rhizoctonia, and Alternaria, some of which act as pathogenic virulent agents and others as secondary pathogens.
英文关键词	Alternate host Fungal diversity Date palm Medicago spp. Wilt disease
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:001222558800001
WOS关键词	MEDICAGO-TRUNCATULA ; FUSARIUM-OXYSPORUM ; RHIZOCTONIA-SOLANI ; PLANT-RESPONSE ; DATE PALM ; RESISTANCE ; PROTECTION ; AGGRESSIVENESS ; COLLECTION ; INFECTION
WOS类目	Agronomy ; Plant Sciences ; Horticulture
WOS研究方向	Agriculture ; Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/403704
推荐引用方式 GB/T 7714	Ben Alaya, Amani,Khiari, Bilel,Ben Slimene, Imen,et al. Diversity and spectrum of pathogenicity of fungi associated with wilt disease of alfalfa in southern Tunisian oases[J],2024,169(4):799-823.
APA	Ben Alaya, Amani.,Khiari, Bilel.,Ben Slimene, Imen.,Chaouachi, Manel.,Badri, Mounawer.,...&Djebali, Naceur.(2024).Diversity and spectrum of pathogenicity of fungi associated with wilt disease of alfalfa in southern Tunisian oases.EUROPEAN JOURNAL OF PLANT PATHOLOGY,169(4),799-823.
MLA	Ben Alaya, Amani,et al."Diversity and spectrum of pathogenicity of fungi associated with wilt disease of alfalfa in southern Tunisian oases".EUROPEAN JOURNAL OF PLANT PATHOLOGY 169.4(2024):799-823.