干旱区生态环境知识资源中心(Arid): Dissemination of antibiotic resistance genes through soil-plant-earthworm continuum in the food production environment

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DOI	10.1016/j.envint.2023.108374
	Dissemination of antibiotic resistance genes through soil-plant-earthworm continuum in the food production environment
	Bhattacharjee, Ananda S.; Phan, Duc; Zheng, Chujing; Ashworth, Daniel; Schmidt, Michael; Men, Yujie; Ferreira, Jorge F. S.; Muir, Gabrielle; Hasan, Nur A.; Ibekwe, Abasiofiok M.
通讯作者	Ibekwe, AM
来源期刊	ENVIRONMENT INTERNATIONAL
ISSN	0160-4120
EISSN	1873-6750
出版年	2024
卷号	183
英文摘要	Treated municipal wastewater (TMW) can provide a reliable source of irrigation water for crops, which is especially important in arid areas where water resources are limited or prone to drought. Nonetheless, TMW may contain residual antibiotics, potentially exposing the crops to these substances. The goal of this study was to investigate the dissemination of antibiotics resistance genes (ARGs) in the soil-plant-earthworm continuum after irrigation of spinach and radish plants with TMW containing trimethoprim, sulfamethoxazole, and sulfapyridine in a greenhouse experiment, followed by feeding of earthworms with harvested plant materials. Our results showed that antibiotic resistance genes (ARGs) were enriched in the soil-plant-earthworm microbiomes irrigated with TMW and TMW spiked with higher concentrations of antibiotics. The number of ARGs and antibioticresistant bacteria (ARB) enrichment varied with plant type, with spinach harboring a significantly higher amount of ARGs and ARB compared to radish. Our data showed that bulk and rhizosphere soils of spinach and radish plants irrigated with MilliQ water, TMW, TMW10, or TMW100 had significant differences in bacterial community (p < 0.001), ARG (p < 0.001), and virulence factor gene (VFG) (p < 0.001) diversities. The abundance of ARGs significantly decreased from bulk soil to rhizosphere to phyllosphere and endosphere. Using metagenome assembled genomes (MAGs), we recovered many bacterial MAGs and a near complete genome (>90 %) of bacterial MAG of genus Leclercia adecarboxylata B from the fecal microbiome of earthworm that was fed harvested radish tubers and spinach leaves grown on TMW10 irrigated waters, and this bacterium has been shown to be an emerging pathogen causing infection in immunocompromised patients that may lead to health complications and death. Therefore, crops irrigated with TMW containing residual antibiotics and ARGs may lead to increased incidences of enrichment of ARB in the soil-plant-earthworm continuum.
英文关键词	Antibiotic resistance genes Antibiotic-resistant bacteria Treated municipal wastewater Water reuse Agriculture Irrigation
类型	Article
语种	英语
开放获取类型	gold
收录类别	SCI-E
WOS记录号	WOS:001138062900001
WOS关键词	WASTE-WATER IRRIGATION ; PERSONAL CARE PRODUCTS ; SEWAGE-SLUDGE ; PHARMACEUTICALS ; GENOME ; IMPACT ; VEGETABLES ; BACTERIA ; FATE ; BIOACCUMULATION
WOS类目	Environmental Sciences
WOS研究方向	Environmental Sciences & Ecology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/403527
推荐引用方式 GB/T 7714	Bhattacharjee, Ananda S.,Phan, Duc,Zheng, Chujing,et al. Dissemination of antibiotic resistance genes through soil-plant-earthworm continuum in the food production environment[J],2024,183.
APA	Bhattacharjee, Ananda S..,Phan, Duc.,Zheng, Chujing.,Ashworth, Daniel.,Schmidt, Michael.,...&Ibekwe, Abasiofiok M..(2024).Dissemination of antibiotic resistance genes through soil-plant-earthworm continuum in the food production environment.ENVIRONMENT INTERNATIONAL,183.
MLA	Bhattacharjee, Ananda S.,et al."Dissemination of antibiotic resistance genes through soil-plant-earthworm continuum in the food production environment".ENVIRONMENT INTERNATIONAL 183(2024).