干旱区生态环境知识资源中心(Arid): Identification and function characterization of BnaBOR4 genes reveal their potential for Brassica napus cultivation under high boron stress

Arid

DOI	10.1016/j.ecoenv.2024.116011
	Identification and function characterization of BnaBOR4 genes reveal their potential for Brassica napus cultivation under high boron stress
	Liu, Ling; Luo, Yu; Ding, Guangda; Wang, Chuang; Cai, Hongmei; Shi, Lei; Xu, Fangsen; Bao, Xiulan; Wang, Sheliang
通讯作者	Wang, SL
来源期刊	ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
ISSN	0147-6513
EISSN	1090-2414
出版年	2024
卷号	271
英文摘要	Boron (B) is essential for plant growth, but toxic in excess. In several countries, soil toxic B levels are always a severe agricultural problem in arid and semi-arid regions. Phytoremediation of excess B containing soil is still in its infancy, while high B tolerant plants with elevated protein abundance of B efflux transporter were successfully established or explored. Brassica napus (B. napus) is one of the most important oil crops. However, B efflux transporters underlying excess B tolerance in B. napus remain unknown. Here, we reported that in Brassicaceae species, B. napus had four homologous genes of Arabidopsis AtBOR4 , which were renamed BnaBOR4.1, BnaBOR4.2, BnaBOR4.3 and BnaBOR4.4. BnaBOR4.1, BnaBOR4.2 and BnaBOR4.3 showed constitutive expression and BnaBOR4.4 appeared to be a pseudogene. BnaBOR4.2 and BnaBOR4.3 were expressed in inner cell layers and BnaBOR4.1 in the outer cell layer in root tip, and all were expressed in vascular tissue in the mature zone. B efflux activity assays in yeast demonstrated that BnaBOR4.1, BnaBOR4.2 and AtBOR4 but not BnaBOR4.3 had comparable levels of B transport activity. Structure-functional analysis between BnaBOR4.3 and BnaBOR4.2 demonstrated that amino acid residue substitution at position 297 (Ala vs Pro) and 427 (Met vs Leu) is critical for the B transport activity. Mutant BnaBOR4.3M427L partially restored the B efflux activity, and both mutants BnaBOR4.3A297P and BnaBOR4.3A297P&M427L fully restored B efflux activity, indicating that the Pro297 residue is critical for their function. Further validation of BnaBOR4 was accomplished by growing transgenic Arabidopsis plants under high B conditions. Taken together, our study identified two functional B efflux genes BnaBOR4.1 and BnaBOR4.2 in B. napus, and a key amino acid residue proline 297 associated with B efflux activity. This study highlights the potential of BanBOR4 genes for B. napus cultivation under high B stress.
英文关键词	Boron toxicity Boron efflux transporter Brassica napus
类型	Article
语种	英语
开放获取类型	gold
收录类别	SCI-E
WOS记录号	WOS:001173308700001
WOS关键词	RHAMNOGALACTURONAN-II ; TOLERANT PLANT ; TRANSPORTER ; ACCUMULATION ; METABOLISM ; TOXICITY ; CELL ; EXPRESSION ; EXPORTER ; SYSTEM
WOS类目	Environmental Sciences ; Toxicology
WOS研究方向	Environmental Sciences & Ecology ; Toxicology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/403475
推荐引用方式 GB/T 7714	Liu, Ling,Luo, Yu,Ding, Guangda,et al. Identification and function characterization of BnaBOR4 genes reveal their potential for Brassica napus cultivation under high boron stress[J],2024,271.
APA	Liu, Ling.,Luo, Yu.,Ding, Guangda.,Wang, Chuang.,Cai, Hongmei.,...&Wang, Sheliang.(2024).Identification and function characterization of BnaBOR4 genes reveal their potential for Brassica napus cultivation under high boron stress.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,271.
MLA	Liu, Ling,et al."Identification and function characterization of BnaBOR4 genes reveal their potential for Brassica napus cultivation under high boron stress".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 271(2024).