干旱区生态环境知识资源中心(Arid): A method for noninvasive individual genotyping of black-footed cat (Felis nigripes)

Arid

DOI	10.1002/ece3.11315
	A method for noninvasive individual genotyping of black-footed cat (Felis nigripes)
	Siziba, Vimbai I.; Scroeder, Michelle M.; Wilson, Beryl; Sliwa, Alexander; Willows-Munro, Sandi
通讯作者	Willows-Munro, S
来源期刊	ECOLOGY AND EVOLUTION
ISSN	2045-7758
出版年	2024
卷号	14 期号:4
英文摘要	The black-footed cat (Felis nigripes) is endemic to the arid regions of southern Africa. One of the world's smallest wild felids, the species occurs at low densities and is secretive and elusive, which makes ecological studies difficult. Genetic data could provide key information such as estimates on population size, sex ratios, and genetic diversity. In this study, we test if microsatellite loci can be successfully amplified from scat samples that could be noninvasively collected from the field. Using 21 blood and scat samples collected from the same individuals, we statistically tested whether nine microsatellites previously designed for use in domestic cats can be used to identify individual black-footed cats. Genotypes recovered from blood and scat samples were compared to assess loss of heterozygosity, allele dropout, and false alleles resulting from DNA degradation or PCR inhibitors present in scat samples. The microsatellite markers were also used to identify individuals from scats collected in the field that were not linked to any blood samples. All nine microsatellites used in this study were amplified successfully and were polymorphic. Microsatellite loci were found to have sufficient discriminatory power to distinguish individuals and identify clones. In conclusion, these molecular markers can be used to monitor populations of wild black-footed cats noninvasively. The genetic data will be able to contribute important information that may be used to guide future conservation initiatives.
英文关键词	allelic dropout fecal DNA Felis nigripes genotyping error microsatellites scats
类型	Article
语种	英语
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:001206931000001
WOS关键词	MICROSATELLITE NULL ALLELES ; MAXIMUM-LIKELIHOOD ; COMPUTER-PROGRAM ; POPULATION-SIZE ; CANIS-RUFUS ; DNA ; CONSERVATION ; PATERNITY ; AMPLIFICATION ; PARENTAGE
WOS类目	Ecology ; Evolutionary Biology
WOS研究方向	Environmental Sciences & Ecology ; Evolutionary Biology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/403456
推荐引用方式 GB/T 7714	Siziba, Vimbai I.,Scroeder, Michelle M.,Wilson, Beryl,et al. A method for noninvasive individual genotyping of black-footed cat (Felis nigripes)[J],2024,14(4).
APA	Siziba, Vimbai I.,Scroeder, Michelle M.,Wilson, Beryl,Sliwa, Alexander,&Willows-Munro, Sandi.(2024).A method for noninvasive individual genotyping of black-footed cat (Felis nigripes).ECOLOGY AND EVOLUTION,14(4).
MLA	Siziba, Vimbai I.,et al."A method for noninvasive individual genotyping of black-footed cat (Felis nigripes)".ECOLOGY AND EVOLUTION 14.4(2024).