干旱区生态环境知识资源中心(Arid): Development and validation of general plasma screening method for performance enhancing drugs in racehorses utilizing liquid chromatography-high-resolution mass spectrometry (LC-HRMS)

Arid

DOI	10.1002/dta.3774
	Development and validation of general plasma screening method for performance enhancing drugs in racehorses utilizing liquid chromatography-high-resolution mass spectrometry (LC-HRMS)
	Lomnicka, Izabela; Dubey, Saurabh; Waller, Pamela; Vora, Dharmikkumar; Dirikolu, Levent
通讯作者	Dirikolu, L
来源期刊	DRUG TESTING AND ANALYSIS
ISSN	1942-7603
EISSN	1942-7611
出版年	2024
英文摘要	The screening of drugs in plasma and urine often requires initial extraction (such as liquid-liquid extraction and solid-phase extraction) before the samples are submitted to instrumental analyses. These extraction procedures are often laborious and time-consuming. In this manuscript, a high-throughput automated assay based on liquid chromatography-high-resolution mass spectrometry (LC-HRMS) suitable for use as an initial testing procedure covering multiple classes of compounds prohibited in horse racing is described. The assay requires a 600-mu L plasma aliquot, which is subjected to solid phase extraction (SPE) using OASIS HLB 96-well SPE with Biotage Extrahera system, evaporation, and reconstitution in a 96-well collection plate. LC-HRMS analyses were carried out on a Thermo Q-Exactive Mass spectrometer coupled with Thermo UHPLC system equipped with Thermo Accela ALS 2.4.0 autosampler linked to ACE Excel column. Drug targets were detected by retention time and accurate mass, with a mass tolerance window of 5 ppm in positive and negative ionization mode. The screening method was validated for over 300 drug targets in a 13-min run. Validation data including sensitivity, specificity, extraction recovery, and precision are presented. As the method employs full-scan mass spectrometry, unlimited number of drug targets can theoretically be incorporated into this method. In this manuscript, a high-throughput automated assay based on liquid chromatography-high-resolution mass spectrometry (LC-HRMS) suitable for use as an initial testing procedure covering multiple classes of compounds prohibited in horse racing was developed. All the target analytes showed good identification capacity yielding good peak shapes separating from other target analytes with no false peak observed at respective retention time of the analytes. As the method employs full-scan mass spectrometry, unlimited number of drug targets can theoretically be incorporated into this method. image
英文关键词	doping equine high resolution mass spectrometry plasma
类型	Article ; Early Access
语种	英语
开放获取类型	Bronze
收录类别	SCI-E
WOS记录号	WOS:001279116400001
WOS关键词	EQUINE PLASMA ; ORBITRAP ; STEROIDS ; MS/MS
WOS类目	Biochemical Research Methods ; Chemistry, Analytical ; Pharmacology & Pharmacy
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/403306
推荐引用方式 GB/T 7714	Lomnicka, Izabela,Dubey, Saurabh,Waller, Pamela,et al. Development and validation of general plasma screening method for performance enhancing drugs in racehorses utilizing liquid chromatography-high-resolution mass spectrometry (LC-HRMS)[J],2024.
APA	Lomnicka, Izabela,Dubey, Saurabh,Waller, Pamela,Vora, Dharmikkumar,&Dirikolu, Levent.(2024).Development and validation of general plasma screening method for performance enhancing drugs in racehorses utilizing liquid chromatography-high-resolution mass spectrometry (LC-HRMS).DRUG TESTING AND ANALYSIS.
MLA	Lomnicka, Izabela,et al."Development and validation of general plasma screening method for performance enhancing drugs in racehorses utilizing liquid chromatography-high-resolution mass spectrometry (LC-HRMS)".DRUG TESTING AND ANALYSIS (2024).