干旱区生态环境知识资源中心(Arid): GhLCYε-3 characterized as a lycopene cyclase gene responding to drought stress in cotton

Arid

DOI	10.1016/j.csbj.2023.12.024
	GhLCYε-3 characterized as a lycopene cyclase gene responding to drought stress in cotton
	Ni, Kesong; Lu, Xuke; Li, Shuyan; Li, Fei; Zhang, Yuexin; Cui, Ruifeng; Fan, Yapeng; Huang, Hui; Chen, Xiugui; Wang, Junjuan; Wang, Shuai; Guo, Lixue; Zhao, Lanjie; He, Yunxin; Ye, Wuwei
通讯作者	Ye, WW
来源期刊	COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
ISSN	2001-0370
出版年	2024
卷号	23 页码:384-395
英文摘要	Drought stress significantly affects crop productivity. Carotenoids are essential photosynthetic pigment for plants, bacteria, and algae, with signaling and antioxidant functions. Lutein is a crucial branch product in the carotenoid synthesis pathway, which effectively improves the stress tolerance of higher plants. lycopene cyclase, a central enzyme for lutein synthesis, holds great significance in regulating lutein production. This research establishes a correlation between lutein content and stress resistance by measuring the drought resistance and lutein content of various cotton materials. To identify which crucial genes are associated with lutein, the lycopene cyclase family (LCYs) was analyzed. The research found that LCYs form a highly conserved family divided into two subfamilies, LCY-epsilon (lycopene epsilon-cyclase) and LCY-beta (lycopene beta-cyclase). Most members of the LCY family contain photoresponsive elements and abscisic acid elements. qRT-PCR demonstrates showed that most genes responded positively to drought stress, and GhLCY epsilon-3 was expressed significantly differently under drought stress. Virus-induced gene silencing (VIGS) assay showed that the content of GhLCY epsilon-3 was significantly increased with MDA and PRO, and the contents of chlorophyll and lutein were significantly decreased in pYL156 plants. The decrease in GhLCY epsilon-3 expression is speculated to lead to reduced lutein content in vivo, resulting in the accumulation of reactive oxygen species (ROS) and decreased drought tolerance. This research enriched the understanding of LCY gene family and lutein function, and provided a new reference for cotton planting in arid areas. Synopsis: Lycopene cyclase plays an important role in enhancing the ability of scavenging ROS and drought resistance of plants.
英文关键词	Carotenoids Cotton Functional verification Lutein Reactive oxygen species (ROS) Lycopene cyclase
类型	Article
语种	英语
开放获取类型	hybrid, Green Published
收录类别	SCI-E
WOS记录号	WOS:001149412300001
WOS关键词	BETA ; OVEREXPRESSION ; IDENTIFICATION ; REVEALS ; PROTEIN ; RNAS
WOS类目	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS研究方向	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/403244
推荐引用方式 GB/T 7714	Ni, Kesong,Lu, Xuke,Li, Shuyan,et al. GhLCYε-3 characterized as a lycopene cyclase gene responding to drought stress in cotton[J],2024,23:384-395.
APA	Ni, Kesong.,Lu, Xuke.,Li, Shuyan.,Li, Fei.,Zhang, Yuexin.,...&Ye, Wuwei.(2024).GhLCYε-3 characterized as a lycopene cyclase gene responding to drought stress in cotton.COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL,23,384-395.
MLA	Ni, Kesong,et al."GhLCYε-3 characterized as a lycopene cyclase gene responding to drought stress in cotton".COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL 23(2024):384-395.