干旱区生态环境知识资源中心(Arid): A sensitive LC-MS/MS methotrexate assay capable of assessing adherence to methotrexate therapy in rheumatoid arthritis

Arid

DOI	10.1515/cclm-2023-0350
	A sensitive LC-MS/MS methotrexate assay capable of assessing adherence to methotrexate therapy in rheumatoid arthritis
	McTaggart, Malcolm P.; Bluett, James; Keevil, Brian G.
通讯作者	McTaggart, MP
来源期刊	CLINICAL CHEMISTRY AND LABORATORY MEDICINE
ISSN	1434-6621
EISSN	1437-4331
出版年	2024
卷号	62 期号:1 页码:111-117
英文摘要	Objectives: To develop a sensitive liquid chromatographytandem mass spectrometry method capable of measuring serum methotrexate in patients with rheumatoid arthritis to assess adherence to drug treatment. Methods: Isotopically labelled internal standard and deionised water were added to sample prior to solid phase extraction using a Waters Oasis Max ion-exchange 96-well plate. Following extraction, samples were analysed by LC-MS/MS on a TQS-micro mass spectrometer. Results: Mean recovery was 107 % for four different concentrations of methotrexate spiked into seven patient samples, whilst post extraction spiking gave a mean recovery of 100 %. Between-batch and within-batch CVs were <= 6 % at three different concentrations of methotrexate in fresh frozen plasma. Mean bias was <5 % for between-batch and within batch analysis at three different weighed in concentrations of methotrexate certified reference material. The lower limit of quantification of the assay was 0.1 nmol/L with linearity up to approximately 100 nmol/L. Dilution linearity studies were used to validate the dilution of patient samples prior to analysis. There was no significant interference in the method from lipaemia, haemolysis or icterus. Conclusions: A sensitive LC-MS/MS assay for methotrexate has been developed and validated. The method has been used to measure methotrexate adherence in patient samples from clinical trials and could be used in future research to assess the ability of the assay as a biofeedback intervention to improve adherence to methotrexate therapy.
英文关键词	adherence mass spectrometry methotrexate rheumatoid arthritis
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:001041852500001
WOS类目	Medical Laboratory Technology
WOS研究方向	Medical Laboratory Technology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/403214
推荐引用方式 GB/T 7714	McTaggart, Malcolm P.,Bluett, James,Keevil, Brian G.. A sensitive LC-MS/MS methotrexate assay capable of assessing adherence to methotrexate therapy in rheumatoid arthritis[J],2024,62(1):111-117.
APA	McTaggart, Malcolm P.,Bluett, James,&Keevil, Brian G..(2024).A sensitive LC-MS/MS methotrexate assay capable of assessing adherence to methotrexate therapy in rheumatoid arthritis.CLINICAL CHEMISTRY AND LABORATORY MEDICINE,62(1),111-117.
MLA	McTaggart, Malcolm P.,et al."A sensitive LC-MS/MS methotrexate assay capable of assessing adherence to methotrexate therapy in rheumatoid arthritis".CLINICAL CHEMISTRY AND LABORATORY MEDICINE 62.1(2024):111-117.