干旱区生态环境知识资源中心(Arid): Variation in pollen viability among Leptochloa crinita accessions: implications for directed crosses

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DOI	10.1080/23818107.2024.2365844
	Variation in pollen viability among Leptochloa crinita accessions: implications for directed crosses
	Dominguez, Deolindo L. E.; Remy, Martina M.; Kozub, Perla C.; Carvajal, Sofia; Cavagnaro, Juan B.; Cavagnaro, Pablo F.
通讯作者	Cavagnaro, PF
来源期刊	BOTANY LETTERS
ISSN	2381-8107
EISSN	2381-8115
出版年	2024
英文摘要	Leptochloa crinita, a native forage grass from American arid regions, is widely used for range grazing and revegetation of degraded lands. Despite its importance, progress in breeding and genetic research has been hindered by the difficulty of achieving sexual crosses between L. crinita plants. We hypothesize that factors associated with pollen quality can influence the effectiveness of directed sexual crosses in this species. Thus, the present study investigated pollen viability and longevity in a genetically diverse collection of 21 L. crinita accessions. Pollen viability was estimated at 0, 30, 60, and 180 minutes after anthers dehiscence (MAAD) by two methods: 1) acetocarmine staining; and 2) in vitro germination of pollen grains in culture media containing 0.4-1.0 M sucrose. The staining method overestimated pollen viability, whereas the in vitro germination assay provided accurate and reliable estimates, being 0.6 M the optimal sucrose concentration in the medium, in terms of germination rates and discriminatory power among the accessions. In all the accessions, pollen viability was highest at 0-30 MAAD, and decayed very rapidly, to reach levels below 5% at 180 MAAD. Significant (p<0.05) and broad variation for pollen viability was found among the accessions, with mean values in the range of 49-89% (0 MAAD), 32-76% (30 MAAD), 24-45% (60 MAAD), and 0.6-4.9% (180 MAAD), allowing the identification of six accessions exhibiting the greatest pollen viabilities. These results suggest 0-30 MAAD and using the selected (best) pollen donors, on emasculated female parentals, as the optimal conditions for attempting sexual crosses in L. crinita.
英文关键词	Leptochloa crinita sexual reproduction pollen viability pollen germination pollen longevity
类型	Article ; Early Access
语种	英语
收录类别	SCI-E
WOS记录号	WOS:001248658800001
WOS关键词	IN-VITRO GERMINATION ; TRICHLORIS-CRINITA ; FORAGE GRASS ; MOLECULAR PHYLOGENY ; HEAT-STRESS ; 3 VARIETIES ; SEED SET ; POACEAE ; LONGEVITY ; CHLORIDOIDEAE
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/403070
推荐引用方式 GB/T 7714	Dominguez, Deolindo L. E.,Remy, Martina M.,Kozub, Perla C.,et al. Variation in pollen viability among Leptochloa crinita accessions: implications for directed crosses[J],2024.
APA	Dominguez, Deolindo L. E.,Remy, Martina M.,Kozub, Perla C.,Carvajal, Sofia,Cavagnaro, Juan B.,&Cavagnaro, Pablo F..(2024).Variation in pollen viability among Leptochloa crinita accessions: implications for directed crosses.BOTANY LETTERS.
MLA	Dominguez, Deolindo L. E.,et al."Variation in pollen viability among Leptochloa crinita accessions: implications for directed crosses".BOTANY LETTERS (2024).