干旱区生态环境知识资源中心(Arid): First genome size assessment and fluorochrome chromosome banding in Argania spinosa (L.) Skeels (Sapotaceae)

Arid

DOI	10.1080/23818107.2023.2280992
	First genome size assessment and fluorochrome chromosome banding in Argania spinosa (L.) Skeels (Sapotaceae)
	Bougoutaia, Youcef; Garnatje, Teresa; Kaid-Harche, Meriem; Siljak-Yakovlev, Sonja; Valles, Joan
通讯作者	Valles, J
来源期刊	BOTANY LETTERS
ISSN	2381-8107
EISSN	2381-8115
出版年	2024
卷号	171 期号:2 页码:159-165
英文摘要	Argania is a monotypic genus belonging to the family Sapotaceae and comprising the only species Argania spinosa. This taxon, endemic to southern Morocco (northern Africa), is important in arid lands. It has a high pastoral value, numerous traditional uses, and it is an important industrial crop for food and cosmetics. A few chromosome counts are available for the species but to date, there is no information on its genome size. Apart from being a parameter useful in many aspects of plant systematic and evolutionary biology, knowing the nuclear DNA amount is a pre-requisite to any genome-sequencing program. In this paper, we present for the first time data on the nuclear DNA content in Argania spinosa, from two populations, and we confirm its chromosome number. Genome size was estimated as 2C = 1.48 pg (1C = 723.72 Mbp) in both studied accessions, revealing a small genome. The chromosome count (2n = 20) revealed that the species has the lowest basic chromosome number (x = 10) in the Sapotaceae, and suggests a descending dysploidy process in the family. The first morphological characterisation of Argania spinosa's karyotype is presented here. In addition, fluorochrome banding, performed with chromomycin A(3) and DAPI, showed the absence of AT-rich regions and the presence of GC-rich regions located in three chromosome pairs, bringing the first molecular cytogenetic data in the genus.
英文关键词	Argania spinosa chromomycin A(3) chromosome number DAPI flow cytometry fluorochrome banding genome size heterochromatin
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:001111685400001
WOS关键词	NUCLEAR-DNA CONTENT ; NUMBERS ; ANGIOSPERMS ; ASTERACEAE ; EVOLUTION
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/403069
推荐引用方式 GB/T 7714	Bougoutaia, Youcef,Garnatje, Teresa,Kaid-Harche, Meriem,et al. First genome size assessment and fluorochrome chromosome banding in Argania spinosa (L.) Skeels (Sapotaceae)[J],2024,171(2):159-165.
APA	Bougoutaia, Youcef,Garnatje, Teresa,Kaid-Harche, Meriem,Siljak-Yakovlev, Sonja,&Valles, Joan.(2024).First genome size assessment and fluorochrome chromosome banding in Argania spinosa (L.) Skeels (Sapotaceae).BOTANY LETTERS,171(2),159-165.
MLA	Bougoutaia, Youcef,et al."First genome size assessment and fluorochrome chromosome banding in Argania spinosa (L.) Skeels (Sapotaceae)".BOTANY LETTERS 171.2(2024):159-165.