干旱区生态环境知识资源中心(Arid): Cytological structures and physiological and biochemical characteristics of covered oat (Avena sativa L.) and naked oat (Avena nuda L.) seeds during high-temperature artificial aging

Arid

DOI	10.1186/s12870-024-05221-2
	Cytological structures and physiological and biochemical characteristics of covered oat (Avena sativa L.) and naked oat (Avena nuda L.) seeds during high-temperature artificial aging
	Yao, Ruirui; Liu, Huan; Wang, Jinglong; Shi, Shangli; Zhao, Guiqin; Zhou, Xiangrui
通讯作者	Liu, H
来源期刊	BMC PLANT BIOLOGY
ISSN	1471-2229
出版年	2024
卷号	24 期号:1
英文摘要	Background Seed aging, a natural and inevitable process occurring during storage. Oats, an annual herb belonging to the Gramineae family and pooideae. In addition to being a healthy food, oats serve as ecological pastures, combating soil salinization and desertification. They also play a role in promoting grassland agriculture and supplementing winter livestock feed. However, the high lipid and fat derivatives contents of oat seeds make them susceptible to deterioration, as fat derivatives are prone to rancidity, affecting oat seed production, storage, development, and germplasm resource utilization. Comparative studies on the effects of aging on physiology and cytological structure in covered and naked oat seeds are limited. Thus, our study aimed to determine the mechanism underlying seed deterioration in artificially aged 'LongYan No. 3' (A. sativa) and 'BaiYan No. 2' (A. nuda) seeds, providing a basis for the physiological evaluation of oat seed aging and serving as a reference for scientifically safe storage and efficient utilization of oats. Results In both oat varieties, superoxide dismutase and catalase activities in seeds showed increasing and decreasing trends, respectively. Variance analysis revealed significant differences and interaction in all measured indicators of oat seeds between the two varieties at different aging times. 'LongYan No. 3' seeds, aged for 24-96 h, exhibited a germination rate of < 30%, Conductivity, malondialdehyde, soluble sugar, and soluble protein levels increased more significantly than the 'BaiYan No. 2'. With prolonged aging leading to cell membrane degradation, reactive oxygen species accumulation, disrupted antioxidant enzyme system, evident embryo cell swelling, and disordered cell arrangement, blocking the nutrient supply route. Simultaneously, severely concentrated chromatin in the nucleus, damaged mitochondrial structure, and impaired energy metabolism were noted, resulting in the loss of 'LongYan No. 3' seed vitality and value. Conversely, 'BaiYan No. 2' seeds showed a germination rate of 73.33% after 96 h of aging, consistently higher antioxidant enzyme activity during aging, normal embryonic cell shape, and existence of the endoplasmic reticulum. Conclusions ROS accumulation and antioxidant enzyme system damage in aged oat seeds, nuclear chromatin condensation, mitochondrial structure damage, nucleic acid metabolism and respiration weakened, oat seed vigor decreased. 'LongYan No. 3' seeds were more severely damaged under artificial aging than 'BaiYan No. 2' seeds, highlighting their heightened susceptibility to aging effects.
英文关键词	Anatomy Artificial aging Biochemistry Physiology Ultrastructure
类型	Article
语种	英语
开放获取类型	Green Accepted, gold
收录类别	SCI-E
WOS记录号	WOS:001244991500001
WOS关键词	PROGRAMMED CELL-DEATH ; ANTIOXIDANT ENZYMES ; STORAGE ; ARABIDOPSIS ; EXPRESSION ; ZINC ; NANOPARTICLES ; MITOCHONDRIAL ; GERMINATION ; MECHANISMS
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/403061
推荐引用方式 GB/T 7714	Yao, Ruirui,Liu, Huan,Wang, Jinglong,et al. Cytological structures and physiological and biochemical characteristics of covered oat (Avena sativa L.) and naked oat (Avena nuda L.) seeds during high-temperature artificial aging[J],2024,24(1).
APA	Yao, Ruirui,Liu, Huan,Wang, Jinglong,Shi, Shangli,Zhao, Guiqin,&Zhou, Xiangrui.(2024).Cytological structures and physiological and biochemical characteristics of covered oat (Avena sativa L.) and naked oat (Avena nuda L.) seeds during high-temperature artificial aging.BMC PLANT BIOLOGY,24(1).
MLA	Yao, Ruirui,et al."Cytological structures and physiological and biochemical characteristics of covered oat (Avena sativa L.) and naked oat (Avena nuda L.) seeds during high-temperature artificial aging".BMC PLANT BIOLOGY 24.1(2024).