干旱区生态环境知识资源中心(Arid): Screening for genetic variability in photosynthetic regulation provides insights into salt performance traits in forage sorghum under salt stress

Arid

DOI	10.1186/s12870-024-05406-9
	Screening for genetic variability in photosynthetic regulation provides insights into salt performance traits in forage sorghum under salt stress
	Amombo, Erick; Gbibar, Maryam; Ashilenje, Dennis S.; Hirich, Abdelaziz; Kouisni, Lamfeddal; Oukarroum, Abdallah; Ghoulam, Cherki; El Gharous, Mohamed; Nilahyane, Abdelaziz
通讯作者	Nilahyane, A
来源期刊	BMC PLANT BIOLOGY
ISSN	1471-2229
出版年	2024
卷号	24 期号:1
英文摘要	Background Sorghum (Sorghum bicolor) is a promising opportunity crop for arid regions of Africa due to its high tolerance to drought and heat stresses. Screening for genetic variability in photosynthetic regulation under salt stress can help to identify target trait combinations essential for sorghum genetic improvement. The primary objective of this study was to identify reliable indicators of photosynthetic performance under salt stress for forage yield within a panel of 18 sorghum varieties from stage 1 (leaf 3) to stage 7 (late flowering to early silage maturity). We dissected the genetic diversity and variability in five stress-sensitive photosynthetic parameters: nonphotochemical chlorophyll fluorescence quenching (NPQ), the electron transport rate (ETR), the maximum potential quantum efficiency of photosystem II (F-V/F-M), the CO2 assimilation rate (A), and the photosynthetic performance based on absorption (PIABS). Further, we investigated potential genes for target phenotypes using a combined approach of bioinformatics, transcriptional analysis, and homologous overexpression. Results The panel revealed polymorphism, two admixed subpopulations, and significant molecular variability between and within population. During the investigated development stages, the PIABS varied dramatically and consistently amongst varieties. Under higher saline conditions, PIABS also showed a significant positive connection with A and dry matter gain. Because PIABS is a measure of plants' overall photosynthetic performance, it was applied to predict the salinity performance index (SPI). The SPI correlated positively with dry matter gain, demonstrating that PIABS could be used as a reliable salt stress performance marker for forage sorghum. Eight rubisco large subunit genes were identified in-silico and validated using qPCR with variable expression across the varieties under saline conditions. Overexpression of Rubisco Large Subunit 8 increased PIABS, altered the OJIP, and growth with an insignificant effect on A. Conclusions These findings provide insights into strategies for enhancing the photosynthetic performance of sorghum under saline conditions for improved photosynthetic performance and potential dry matter yield. The integration of molecular approaches, guided by the identified genetic variability, holds promise for genetically breeding sorghum tailored to thrive in arid and saline environments, contributing to sustainable agricultural practices.
英文关键词	Genetic diversity Growth stage Photosynthetic performance index SbrbcL8 Yield Salt performance index
类型	Article
语种	英语
开放获取类型	gold
收录类别	SCI-E
WOS记录号	WOS:001272959100002
WOS关键词	CHLOROPHYLL FLUORESCENCE ; SOIL-SALINITY ; WEB SERVER ; SOFTWARE ; PLANTS ; RESOURCES ; GENOTYPES ; LIGHT
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/403056
推荐引用方式 GB/T 7714	Amombo, Erick,Gbibar, Maryam,Ashilenje, Dennis S.,et al. Screening for genetic variability in photosynthetic regulation provides insights into salt performance traits in forage sorghum under salt stress[J],2024,24(1).
APA	Amombo, Erick.,Gbibar, Maryam.,Ashilenje, Dennis S..,Hirich, Abdelaziz.,Kouisni, Lamfeddal.,...&Nilahyane, Abdelaziz.(2024).Screening for genetic variability in photosynthetic regulation provides insights into salt performance traits in forage sorghum under salt stress.BMC PLANT BIOLOGY,24(1).
MLA	Amombo, Erick,et al."Screening for genetic variability in photosynthetic regulation provides insights into salt performance traits in forage sorghum under salt stress".BMC PLANT BIOLOGY 24.1(2024).