干旱区生态环境知识资源中心(Arid): Transcriptome profiles reveal response mechanisms and key role of PsNAC1 in Pinus sylvestris var. mongolica to drought stress

Arid

DOI	10.1186/s12870-024-05051-2
	Transcriptome profiles reveal response mechanisms and key role of PsNAC1 in Pinus sylvestris var. mongolica to drought stress
	Zhou, Chengcheng; Bo, Wenhao; El-Kassaby, Yousry A.; Li, Wei
通讯作者	Li, W
来源期刊	BMC PLANT BIOLOGY
ISSN	1471-2229
出版年	2024
卷号	24 期号:1
英文摘要	Background Drought stress severely impedes plant growth, and only a limited number of species exhibit long-term resistance to such conditions. Pinus sylvestris var. mongolica, a dominant tree species in arid and semi-arid regions of China, exhibits strong drought resistance and plays a crucial role in the local ecosystem. However, the molecular mechanisms underlying this resistance remain poorly understood. Results Here, we conducted transcriptome sequence and physiological indicators analysis of needle samples during drought treatment and rehydration stages. De-novo assembly yielded approximately 114,152 unigenes with an N50 length of 1,363 bp. We identified 6,506 differentially expressed genes (DEGs), with the majority being concentrated in the heavy drought stage (4,529 DEGs). Functional annotation revealed enrichment of drought-related GO terms such as response to water (GO:0009415: enriched 108 genes) and response to water deprivation (GO:0009414: enriched 106 genes), as well as KEGG categories including MAPK signaling pathway (K04733: enriched 35 genes) and monoterpenoid biosynthesis (K21374: enriched 27 genes). Multiple transcription factor families and functional protein families were differentially expressed during drought treatment. Co-expression network analysis identified a potential drought regulatory network between cytochrome P450 genes (Unigene4122_c1_g1) and a core regulatory transcription factor Unigene9098_c3_g1 (PsNAC1) with highly significant expression differences. We validated PsNAC1 overexpression in Arabidopsis and demonstrated enhanced drought resistance. Conclusions These findings provide insight into the molecular basis of drought resistance in P. sylvestris var. mongolica and lay the foundation for further exploration of its regulatory network.
英文关键词	Pinus sylvestris var. mongolica NAC transcription factor Transcriptome Drought stress
类型	Article
语种	英语
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:001210623800003
WOS关键词	ABIOTIC STRESS ; BIOSYNTHESIS ; NAC ; TOLERANCE ; GENOME ; METHYLERYTHRITOL ; CYTOSCAPE ; RECOVERY ; SEQUENCE ; DEFENSE
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/403055
推荐引用方式 GB/T 7714	Zhou, Chengcheng,Bo, Wenhao,El-Kassaby, Yousry A.,et al. Transcriptome profiles reveal response mechanisms and key role of PsNAC1 in Pinus sylvestris var. mongolica to drought stress[J],2024,24(1).
APA	Zhou, Chengcheng,Bo, Wenhao,El-Kassaby, Yousry A.,&Li, Wei.(2024).Transcriptome profiles reveal response mechanisms and key role of PsNAC1 in Pinus sylvestris var. mongolica to drought stress.BMC PLANT BIOLOGY,24(1).
MLA	Zhou, Chengcheng,et al."Transcriptome profiles reveal response mechanisms and key role of PsNAC1 in Pinus sylvestris var. mongolica to drought stress".BMC PLANT BIOLOGY 24.1(2024).