干旱区生态环境知识资源中心(Arid): Integrated transcriptomic and metabolomic analyses reveals anthocyanin biosynthesis in leaf coloration of quinoa (Chenopodium quinoa Willd.)

Arid

DOI	10.1186/s12870-024-04821-2
	Integrated transcriptomic and metabolomic analyses reveals anthocyanin biosynthesis in leaf coloration of quinoa (Chenopodium quinoa Willd.)
	Zhang, Min; Li, Yueyou; Wang, Junling; Shang, Shaopu; Wang, Hongxia; Yang, Xinlei; Lu, Chuan; Wang, Mei; Sun, Xinbo; Liu, Xiaoqing; Wang, Xiaoxia; Wei, Boxiang; Lv, Wei; Mu, Guojun
通讯作者	Mu, GJ
来源期刊	BMC PLANT BIOLOGY
ISSN	1471-2229
出版年	2024
卷号	24 期号:1
英文摘要	Background Quinoa leaves demonstrate a diverse array of colors, offering a potential enhancement to landscape aesthetics and the development of leisure-oriented sightseeing agriculture in semi-arid regions. This study utilized integrated transcriptomic and metabolomic analyses to investigate the mechanisms underlying anthocyanin synthesis in both emerald green and pink quinoa leaves. Results Integrated transcriptomic and metabolomic analyses indicated that both flavonoid biosynthesis pathway (ko00941) and anthocyanin biosynthesis pathway (ko00942) were significantly associated with anthocyanin biosynthesis. Differentially expressed genes (DEGs) and differentially accumulated metabolites (DAMs) were analyzed between the two germplasms during different developmental periods. Ten DEGs were verified using qRT-PCR, and the results were consistent with those of the transcriptomic sequencing. The elevated expression of phenylalanine ammonia-lyase (PAL), chalcone synthase (CHS), 4-coumarate CoA ligase (4CL) and Hydroxycinnamoyltransferase (HCT), as well as the reduced expression of flavanone 3-hydroxylase (F3H) and Flavonol synthase (FLS), likely cause pink leaf formation. In addition, bHLH14, WRKY46, and TGA indirectly affected the activities of CHS and 4CL, collectively regulating the levels of cyanidin 3-O-(3'', 6''-O-dimalonyl) glucoside and naringenin. The diminished expression of PAL, 4CL, and HCT decreased the formation of cyanidin-3-O-(6-O-malonyl-2-O-glucuronyl) glucoside, leading to the emergence of emerald green leaves. Moreover, the lowered expression of TGA and WRKY46 indirectly regulated 4CL activity, serving as another important factor in maintaining the emerald green hue in leaves N1, N2, and N3. Conclusion These findings establish a foundation for elucidating the molecular regulatory mechanisms governing anthocyanin biosynthesis in quinoa leaves, and also provide some theoretical basis for the development of leisure and sightseeing agriculture.
英文关键词	DEGs DAMs TFs Integrated transcriptomic and metabolomic analyses qRT-PCR
类型	Article
语种	英语
开放获取类型	Green Submitted, gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:001190598800001
WOS关键词	CHALCONE SYNTHASE GENES ; FLAVONOID ACCUMULATION ; FLOWER COLOR ; EXPRESSION ; TRANSPORT ; TT2
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/403049
推荐引用方式 GB/T 7714	Zhang, Min,Li, Yueyou,Wang, Junling,et al. Integrated transcriptomic and metabolomic analyses reveals anthocyanin biosynthesis in leaf coloration of quinoa (Chenopodium quinoa Willd.)[J],2024,24(1).
APA	Zhang, Min.,Li, Yueyou.,Wang, Junling.,Shang, Shaopu.,Wang, Hongxia.,...&Mu, Guojun.(2024).Integrated transcriptomic and metabolomic analyses reveals anthocyanin biosynthesis in leaf coloration of quinoa (Chenopodium quinoa Willd.).BMC PLANT BIOLOGY,24(1).
MLA	Zhang, Min,et al."Integrated transcriptomic and metabolomic analyses reveals anthocyanin biosynthesis in leaf coloration of quinoa (Chenopodium quinoa Willd.)".BMC PLANT BIOLOGY 24.1(2024).