干旱区生态环境知识资源中心(Arid): Molecular and agro-morphological characterization of new barley genotypes in arid environments

Arid

DOI	10.1186/s12896-024-00861-6
	Molecular and agro-morphological characterization of new barley genotypes in arid environments
	Elshafei, Adel A.; Ibrahim, Eid I.; Abdellatif, Kamal F.; Salem, Abd El-Azeem K.; Moustafa, Khaled A.; Al-Doss, Abdullah A.; Migdadi, Hussein M.; Hussien, Amal M.; Soufan, Walid; Abd El Rahman, Taha; Eldemery, Samah M.
通讯作者	Elshafei, AA
来源期刊	BMC BIOTECHNOLOGY
EISSN	1472-6750
出版年	2024
卷号	24 期号:1
英文摘要	Background Genetic diversity, population structure, agro-morphological traits, and molecular characteristics, are crucial for either preserving genetic resources or developing new cultivars. Due to climate change, water availability for agricultural use is progressively diminishing. This study used 100 molecular markers (25 TRAP, 22 SRAP, 23 ISTR, and 30 SSR). Additionally, 15 morphological characteristics were utilized to evaluate the optimal agronomic traits of 12 different barley genotypes under arid conditions.Results Substantial variations, ranging from significant to highly significant, were observed in the 15 agromorphological parameters evaluated among the 12 genotypes. The KSU-B101 barley genotype demonstrated superior performance in five specific traits: spike number per plant, 100-grain weight, spike number per square meter, harvest index, and grain yield. These results indicate its potential for achieving high yields in arid regions. The Sahrawy barley genotype exhibited the highest values across five parameters, namely leaf area, spike weight per plant, spike length, spike weight per square meter, and biological yield, making it a promising candidate for animal feed. The KSU-B105 genotype exhibited early maturity and a high grain count per spike, which reflects its early maturity and ability to produce a high number of grains per spike. This suggests its suitability for both animal feed and human food in arid areas. Based on marker data, the molecular study found that the similarity coefficients between the barley genotypes ranged from 0.48 to 0.80, with an average of 0.64. The dendrogram constructed from these data revealed three distinct clusters with a similarity coefficient of 0.80. Notably, the correlation between the dendrogram and its similarity matrix was high (0.903), indicating its accuracy in depicting the genetic relationships. The combined analysis revealed a moderate correlation between the morphological and molecular analysis, suggesting alignment between the two characterization methods.Results Substantial variations, ranging from significant to highly significant, were observed in the 15 agromorphological parameters evaluated among the 12 genotypes. The KSU-B101 barley genotype demonstrated superior performance in five specific traits: spike number per plant, 100-grain weight, spike number per square meter, harvest index, and grain yield. These results indicate its potential for achieving high yields in arid regions. The Sahrawy barley genotype exhibited the highest values across five parameters, namely leaf area, spike weight per plant, spike length, spike weight per square meter, and biological yield, making it a promising candidate for animal feed. The KSU-B105 genotype exhibited early maturity and a high grain count per spike, which reflects its early maturity and ability to produce a high number of grains per spike. This suggests its suitability for both animal feed and human food in arid areas. Based on marker data, the molecular study found that the similarity coefficients between the barley genotypes ranged from 0.48 to 0.80, with an average of 0.64. The dendrogram constructed from these data revealed three distinct clusters with a similarity coefficient of 0.80. Notably, the correlation between the dendrogram and its similarity matrix was high (0.903), indicating its accuracy in depicting the genetic relationships. The combined analysis revealed a moderate correlation between the morphological and molecular analysis, suggesting alignment between the two characterization methods. Conclusions The morphological and molecular analyses of the 12 barley genotypes in this study effectively revealed the varied genetic characteristics of their agro-performance in arid conditions. KSU-B101, Sahrawy, and KSU-B105 have emerged as promising candidates for different agricultural applications in arid regions. Further research on these genotypes could reveal their full potential for breeding programs.
英文关键词	Barley Biodiversity Molecular markers STRUCTURE PCoA
类型	Article
语种	英语
开放获取类型	Green Accepted, gold
收录类别	SCI-E
WOS记录号	WOS:001243935200001
WOS关键词	GENETIC DIVERSITY ; MICROSATELLITE MARKERS ; L. ; SSR ; VARIABILITY ; RAPD ; MAP ; CULTIVARS ; YIELD ; ISSR
WOS类目	Biotechnology & Applied Microbiology
WOS研究方向	Biotechnology & Applied Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/403042
推荐引用方式 GB/T 7714	Elshafei, Adel A.,Ibrahim, Eid I.,Abdellatif, Kamal F.,et al. Molecular and agro-morphological characterization of new barley genotypes in arid environments[J],2024,24(1).
APA	Elshafei, Adel A..,Ibrahim, Eid I..,Abdellatif, Kamal F..,Salem, Abd El-Azeem K..,Moustafa, Khaled A..,...&Eldemery, Samah M..(2024).Molecular and agro-morphological characterization of new barley genotypes in arid environments.BMC BIOTECHNOLOGY,24(1).
MLA	Elshafei, Adel A.,et al."Molecular and agro-morphological characterization of new barley genotypes in arid environments".BMC BIOTECHNOLOGY 24.1(2024).