干旱区生态环境知识资源中心(Arid): Development, validation and application of an ion-pair reversed-phase liquid chromatography-tandem mass spectrometry method for the quantification of nusinersen

Arid

DOI	10.4155/bio-2023-0218
	Development, validation and application of an ion-pair reversed-phase liquid chromatography-tandem mass spectrometry method for the quantification of nusinersen
	Zhang, Xiao; Sha, Chunjie; Zhang, Wei; Zhao, Fengjuan; Zhu, Mingli; Leng, Guangyi; Liu, Wanhui
通讯作者	Liu, WH
来源期刊	BIOANALYSIS
ISSN	1757-6180
EISSN	1757-6199
出版年	2024
卷号	16 期号:5
英文摘要	Background: The fully phosphorothioate-modified oligonucleotide (OGN) nusinersen has low ionization efficiency in the negative ion mode, resulting in a low mass spectrometry response. There have been no relevant reports on developing a LC-MS method for the determination of nusinersen by optimizing mobile phase composition. Materials & methods: Mobile phase additives comprised of 15 mM triethylamine/25 mM 1,1,1,3,3,3-hexafluoro-2-propanol with a pH of 9.6. Nusinersen was extracted from plasma using Oasis (R) HLB solid-phase extraction (Waters, MA, USA). Results & conclusion: By adjusting the pH of the mobile phase to 9.6 by optimizing the type and concentration of ion-pair reagents, a high mass spectrometry response was obtained. The developed method was applied to nusinersen and met the requirements for the pharmacokinetic study of nusinersen in rabbits.
英文关键词	IP-RP-LC/MS/MS nusinersen phosphorothioate modification quantitation
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:001159689800001
WOS关键词	RAT PLASMA ; LC-MS ; ANTISENSE OLIGONUCLEOTIDES ; BIOANALYTICAL METHOD ; METABOLITE ; PHARMACOKINETICS ; URINE ; MS/MS
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/403007
推荐引用方式 GB/T 7714	Zhang, Xiao,Sha, Chunjie,Zhang, Wei,et al. Development, validation and application of an ion-pair reversed-phase liquid chromatography-tandem mass spectrometry method for the quantification of nusinersen[J],2024,16(5).
APA	Zhang, Xiao.,Sha, Chunjie.,Zhang, Wei.,Zhao, Fengjuan.,Zhu, Mingli.,...&Liu, Wanhui.(2024).Development, validation and application of an ion-pair reversed-phase liquid chromatography-tandem mass spectrometry method for the quantification of nusinersen.BIOANALYSIS,16(5).
MLA	Zhang, Xiao,et al."Development, validation and application of an ion-pair reversed-phase liquid chromatography-tandem mass spectrometry method for the quantification of nusinersen".BIOANALYSIS 16.5(2024).