干旱区生态环境知识资源中心(Arid): Biosignature Molecules Accumulate and Persist in Evaporitic Brines: Implications for Planetary Exploration

Arid

DOI	10.1089/ast.2023.0122
	Biosignature Molecules Accumulate and Persist in Evaporitic Brines: Implications for Planetary Exploration
	Pozarycki, Chad; Seaton, Kenneth M.; C. Vincent, Emily; Sanders, Carlie Novak; Nunez, Nickie; Castillo, Mariah; Ingall, Ellery; Klempay, Benjamin; Pontefract, Alexandra; Fisher, Luke A.; Paris, Emily R.; Buessecker, Steffen; Alansson, Nikolas B.; Carr, Christopher E.; Doran, Peter T.; Bowman, Jeff S.; Schmidt, Britney E.; Stockton, Amanda M.
通讯作者	Stockton, AM
来源期刊	ASTROBIOLOGY
ISSN	1531-1074
EISSN	1557-8070
出版年	2024
卷号	24 期号:8 页码:795-812
英文摘要	The abundance of potentially habitable hypersaline environments in our solar system compels us to understand the impacts of high-salt matrices and brine dynamics on biosignature detection efforts. We identified and quantified organic compounds in brines from South Bay Salt Works (SBSW), where evapoconcentration of ocean water enables exploration of the impact of NaCl- and MgCl2-dominated brines on the detection of potential biosignature molecules. In SBSW, organic biosignature abundance and distribution are likely influenced by evapoconcentration, osmolyte accumulation, and preservation effects. Bioluminescence assays show that adenosine triphosphate (ATP) concentrations are higher in NaCl-rich, low water activity (a(w)) samples (<0.85) from SBSW. This is consistent with the accumulation and preservation of ATP at low a(w) as described in past laboratory studies. The water-soluble small organic molecule inventory was determined by using microchip capillary electrophoresis paired with high-resolution mass spectrometry (CE-HRMS). We analyzed the relative distribution of proteinogenic amino acids with a recently developed quantitative method using CE-separation and laser-induced fluorescence (LIF) detection of amino acids in hypersaline brines. Salinity trends for dissolved free amino acids were consistent with amino acid residue abundance determined from the proteome of the microbial community predicted from metagenomic data. This highlights a tangible connection up and down the -omics ladder across changing geochemical conditions. The detection of water-soluble organic compounds, specifically proteinogenic amino acids at high abundance (>7 mM) in concentrated brines, demonstrates that potential organic biomarkers accumulate at hypersaline sites and suggests the possibility of long-term preservation. The detection of such molecules in high abundance when using diverse analytical tools appropriate for spacecraft suggests that life detection within hypersaline environments, such as evaporates on Mars and the surface or subsurface brines of ocean world Europa, is plausible and argues such environments should be a high priority for future exploration.
英文关键词	Salts Analytical chemistry Amino acids Biosignatures Capillary electrophoresis Preservation
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:001293660900006
WOS关键词	DISSOLVED ORGANIC-MATTER ; HYPER-ARID ZONE ; AMINO-ACIDS ; WATER ACTIVITY ; CAPILLARY-ELECTROPHORESIS ; MICROBIAL DIVERSITY ; EQUATORIAL PACIFIC ; HYPERSALINE BASIN ; MASS-SPECTROMETRY ; LIFE
WOS类目	Astronomy & Astrophysics ; Biology ; Geosciences, Multidisciplinary
WOS研究方向	Astronomy & Astrophysics ; Life Sciences & Biomedicine - Other Topics ; Geology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/402961
推荐引用方式 GB/T 7714	Pozarycki, Chad,Seaton, Kenneth M.,C. Vincent, Emily,et al. Biosignature Molecules Accumulate and Persist in Evaporitic Brines: Implications for Planetary Exploration[J],2024,24(8):795-812.
APA	Pozarycki, Chad.,Seaton, Kenneth M..,C. Vincent, Emily.,Sanders, Carlie Novak.,Nunez, Nickie.,...&Stockton, Amanda M..(2024).Biosignature Molecules Accumulate and Persist in Evaporitic Brines: Implications for Planetary Exploration.ASTROBIOLOGY,24(8),795-812.
MLA	Pozarycki, Chad,et al."Biosignature Molecules Accumulate and Persist in Evaporitic Brines: Implications for Planetary Exploration".ASTROBIOLOGY 24.8(2024):795-812.