干旱区生态环境知识资源中心(Arid): Enhancing Pisum sativum growth and symbiosis under heat stress: the synergistic impact of co-inoculated bacterial consortia and ACC deaminase-lacking Rhizobium

Arid

DOI	10.1007/s00203-024-03943-3
	Enhancing Pisum sativum growth and symbiosis under heat stress: the synergistic impact of co-inoculated bacterial consortia and ACC deaminase-lacking Rhizobium
	Ben Gaied, Roukaya; Sbissi, Imed; Tarhouni, Mohamed; Brigido, Clarisse
通讯作者	Brígido, C
来源期刊	ARCHIVES OF MICROBIOLOGY
ISSN	0302-8933
EISSN	1432-072X
出版年	2024
卷号	206 期号:5
英文摘要	The 1-aminocyclopropane-1-carboxylate (ACC) deaminase is a crucial bacterial trait, yet it is not widely distributed among rhizobia. Hence, employing a co-inoculation approach that combines selected plant growth-promoting bacteria with compatible rhizobial strains, especially those lacking ACC deaminase, presents a practical solution to alleviate the negative effects of diverse abiotic stresses on legume nodulation. Our objective was to explore the efficacy of three non-rhizobial endophytes, Phyllobacterium salinisoli (PH), Starkeya sp. (ST) and Pseudomonas turukhanskensis (PS), isolated from native legumes grown in Tunisian arid regions, in improving the growth of cool-season legume and fostering symbiosis with an ACC deaminase-lacking rhizobial strain under heat stress. Various combinations of these endophytes (ST + PS, ST + PH, PS + PH, and ST + PS + PH) were co-inoculated with Rhizobium leguminosarum 128C53 or its Delta acdS mutant derivative on Pisum sativum plants exposed to a two-week heat stress period. Our findings revealed that the absence of ACC deaminase activity negatively impacted both pea growth and symbiosis under heat stress. Nevertheless, these detrimental effects were successfully mitigated in plants co-inoculated with Delta acdS mutant strain and specific non-rhizobial endophytes consortia. Our results indicated that heat stress significantly altered the phenolic content of pea root exudates. Despite this, there was no impact on IAA production. Interestingly, these changes positively influenced biofilm formation in consortia containing the mutant strain, indicating synergistic bacteria-bacteria interactions. Additionally, no positive effects were observed when these endophytic consortia were combined with the wild-type strain. This study highlights the potential of non-rhizobial endophytes to improve symbiotic performance of rhizobial strains lacking genetic mechanisms to mitigate stress effects on their legume host, holding promising potential to enhance the growth and yield of targeted legumes by boosting symbiosis.
英文关键词	Nodule-associated endophytes Root exudation Bacterial consortia Abiotic stress Nitrogen fixation Nodulation
类型	Article
语种	英语
开放获取类型	hybrid
收录类别	SCI-E
WOS记录号	WOS:001197456900003
WOS关键词	PLANT-GROWTH ; ROOT-NODULES ; NITROGEN-FIXATION ; NODULATION ; STRAINS ; ASSOCIATIONS ; ENHANCEMENT ; TEMPERATURE ; PROMOTION ; GENE
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/402934
推荐引用方式 GB/T 7714	Ben Gaied, Roukaya,Sbissi, Imed,Tarhouni, Mohamed,et al. Enhancing Pisum sativum growth and symbiosis under heat stress: the synergistic impact of co-inoculated bacterial consortia and ACC deaminase-lacking Rhizobium[J],2024,206(5).
APA	Ben Gaied, Roukaya,Sbissi, Imed,Tarhouni, Mohamed,&Brigido, Clarisse.(2024).Enhancing Pisum sativum growth and symbiosis under heat stress: the synergistic impact of co-inoculated bacterial consortia and ACC deaminase-lacking Rhizobium.ARCHIVES OF MICROBIOLOGY,206(5).
MLA	Ben Gaied, Roukaya,et al."Enhancing Pisum sativum growth and symbiosis under heat stress: the synergistic impact of co-inoculated bacterial consortia and ACC deaminase-lacking Rhizobium".ARCHIVES OF MICROBIOLOGY 206.5(2024).